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Immunofluorescent analysis of Dengue Virus Type 2 NS5 showing staining in the nucleus of BHK-21 cells. BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde and stained using a Dengue Virus Type 2 NS5 monoclonal antibody (Product # MA5-17295) diluted at 1:2000. Blue: Hoechst 33342 staining.
|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide contains a sequence corresponding to a region within amino acids 829 and 900 of NS5 (Dengue Virus 2)|
|Storage buffer||PBS, pH 7|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17295 targets Dengue Virus 2 NS5 in ICC, IF, and WB applications and shows reactivity with Virus samples.
The MA5-17295 immunogen is synthetic peptide contains a sequence corresponding to a region within amino acids 829 and 900 of NS5 (Dengue Virus 2).
Dengue fever and dengue hemorrhagic fever are acute febrile diseases, found in the tropics and Africa. Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. Dengue is transmitted to humans by the Aedes aegypti (rarely Aedes albopictus) mosquito, which feeds during the day.
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