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|Tested species reactivity||Mammal|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length human Desmin.|
|Storage buffer||PBS, pH 7.2, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:75-1:150|
|Immunohistochemistry (Paraffin) (IHC (P))||1:75-1:150|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27003 detects Desmin from all mammal samples.
PA1-27003 has been successfully used in immunofluorescence, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot procedures. Antigen retrieval is required for immunostaining.
The PA1-27003 immunogen is full length human protein.
Desmin is a 53 kDa intermediate filament protein expressed in cardiac, skeletal, and smooth muscle cells, as well as lung tissue. In skeletal and cardiac muscle cells, desmin is localized at the Z-disk region and the intercalated disk. In smooth muscle tissue, desmin is expressed throughout the cells. During development, levels of desmin expression vary, being present at low levels in the early stages of myoblast replication and increasing during terminal differentiation. Unlike vimentin, desmin is expressed in both differentiating and mature muscle cells. Inhibiting desmin expression blocks myoblast fusion and myotube formation, suggesting that desmin is crucial during myogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CMD1I; CSM2; DES; Desmin; FLJ12025; intermediate filament protein; muscle-specific intermediate filament desmin; mutant desmin p.K241E
BOS_2078; CSM1; CSM2; DES; LGMD2R