|Tested species reactivity||Dog, Chicken, Hamster, Human, Mouse, Pig, Rabbit, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||A cytoskeletal extract of chicken gizzard.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100 - 1:200|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Desmin is a 53 kDa intermediate filament protein expressed in cardiac, skeletal, and smooth muscle cells, as well as lung tissue. In skeletal and cardiac muscle cells, desmin is localized at the Z-disk region and the intercalated disk. In smooth muscle tissue, desmin is expressed throughout the cells. During development, levels of desmin expression vary, being present at low levels in the early stages of myoblast replication and increasing during terminal differentiation. Unlike vimentin, desmin is expressed in both differentiating and mature muscle cells. Inhibiting desmin expression blocks myoblast fusion and myotube formation, suggesting that desmin is crucial during myogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Stainless steel ions stimulate increased thrombospondin-1-dependent TGF-beta activation by vascular smooth muscle cells: implications for in-stent restenosis.
MA1-12788 was used in western blot to investigate the effect of stainless steel ions on the activity of vascular smooth muscle cells
|Pallero MA,Talbert Roden M,Chen YF,Anderson PG,Lemons J,Brott BC,Murphy-Ullrich JE||Journal of vascular research (47:309)||2010|