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Immunohistochemistry analysis of Desmocollin-2/3 showing staining in the membrane of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Desmocollin-2/3 monoclonal antibody (326200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human desmocollin-2 extracellular domain.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||5-10 ug|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells.
32-6200 was used in immunocytochemistry and western blot to study the role of desmocollin-2 in adhesive strength and cytoskeleton architecture in esophageal squamous cell carcinoma
|Fang WK,Liao LD,Zeng FM,Zhang PX,Wu JY,Shen J,Xu LY,Li EM||Molecular medicine reports (10:2358)||2014|
|Human||Not Cited||Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.||Raiko L,Siljamäki E,Mahoney MG,Putaala H,Suominen E,Peltonen J,Peltonen S||Experimental dermatology (21:586)||2012|
130-kD pemphigus vulgaris antigen; ARVC10; ARVD10; cadherin family member 2; cadherin family member 3; cadherin family member 5; cadherin family member 6; CDHF5; CDHF6; CMD1BB; desmocollin-4; desmoglein-2; desmoglein-3; desmosomal glycoprotein II/III; pemphigus vulgaris antigen; PVA
ARVD11; CDHF2; CDHF3; DG2; DGII/III; DSC; DSC1; DSC2; DSC3; DSC4; HT-CP