Immunohistochemistry analysis of Desmoglein-1 showing staining in the cytoplasm and membrane of paraffin-embedded human skin tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Desmoglein-1 monoclonal antibody (326000) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human desmoglein-1 intracellular domain.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||20-50 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||5-10 ug|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Early dental epithelial transcription factors distinguish ameloblastoma from keratocystic odontogenic tumor.
32-6000 was used in immunohistochemistry to evaluate early dental epithelial transcription factors as a diagnostic marker for ameloblastoma and keratocystic odontogenic tumor
|Heikinheimo K,Kurppa KJ,Laiho A,Peltonen S,Berdal A,Bouattour A,Ruhin B,Catón J,Thesleff I,Leivo I,Morgan PR||Journal of dental research (94:101)||2015|
Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis.
32-6000 was used in western blot to assess if the EphA2/ephrin-A1 signaling axis is altered in psoriasis.
|Gordon K,Kochkodan JJ,Blatt H,Lin SY,Kaplan N,Johnston A,Swindell WR,Hoover P,Schlosser BJ,Elder JT,Gudjonsson JE,Getsios S||The Journal of investigative dermatology (133:712)||2013|
Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection.
32-6000 was used in immunohistochemistry to examine the contribution of the desmosomal compartment to gastroesophageal reflux disease.
|Wex T,Kuester D,Mönkemüller K,Stahr A,Fry LC,Kandulski A,Kropf S,Roessner A,Malfertheiner P||Human pathology (43:1745)||2012|
Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa.
32-6000 was used in immunohistochemistry to examine the role of desmosomal proteins in gastro-oesophageal reflux disease.
|Wex T,Mönkemüller K,Stahr A,Kuester D,Fry LC,Völkel S,Kandulski A,Roessner A,Malfertheiner P||Histopathology (60:405)||2012|
Ligand targeting of EphA2 enhances keratinocyte adhesion and differentiation via desmoglein 1.
32-6000 was used in western blot to suggest that EphA2-expressing keratinocytes respond to ephrin-A1-based cues from their neighbors to facilitate entry into a terminal differentiation pathway
|Lin S,Gordon K,Kaplan N,Getsios S||Molecular biology of the cell (21:3902)||2010|
|Human||Not Cited||Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.||Raiko L,Siljamäki E,Mahoney MG,Putaala H,Suominen E,Peltonen J,Peltonen S||Experimental dermatology (21:586)||2012|
|Human||Not Cited||EGFR regulation of epidermal barrier function.||Tran QT,Kennedy LH,Leon Carrion S,Bodreddigari S,Goodwin SB,Sutter CH,Sutter TR||Physiological genomics (44:455)||2012|
|Human||Not Cited||Human eccrine sweat gland cells can reconstitute a stratified epidermis.||Biedermann T,Pontiggia L,Böttcher-Haberzeth S,Tharakan S,Braziulis E,Schiestl C,Meuli M,Reichmann E||The Journal of investigative dermatology (130:1996)||2010|
|Not Applicable||Not Cited||
P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells.
32-6000 was used in immunoprecipitation to test if p120ctn associates with Dsg1 and Dsg3 in keratinocytes
|Kanno M,Aoyama Y,Isa Y,Yamamoto Y,Kitajima Y||The Journal of dermatology (35:317)||2008|
New skin-equivalent model from de-epithelialized amnion membrane.
32-6000 was used in immunohistochemistry - paraffin section to develop a living skin-equivalent from de-epithelialized amnion membrane
|Yang L,Shirakata Y,Shudou M,Dai X,Tokumaru S,Hirakawa S,Sayama K,Hamuro J,Hashimoto K||Cell and tissue research (326:69)||2006|