Western blot analysis of Desmoglein-2 was performed by loading 30 ug of HEK-293 (lane1), A549 (lane2), A-431 (lane3), and K562 (lane4) cell lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (NP0321BOX), XCell SureLock™ Electrophoresis System (EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and Pierce™ Power Blotter System (22834). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Desmoglein-2 was detected at ~122 kDa using Desmoglein-2 Mouse Monoclonal Antibody (326100) at 1-2 ug/ml in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (626520) at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (WP20005).
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||5-10 ug|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Desmoglein 2 depletion leads to increased migration and upregulation of the chemoattractant secretoneurin in melanoma cells.
32-6100 was used in immunocytochemistry and western blot to determine the role of desmoglein 2 in melanoma cells
|Peitsch WK,Doerflinger Y,Fischer-Colbrie R,Huck V,Bauer AT,Utikal J,Goerdt S,Schneider SW||PloS one (9:null)||2014|
Differential downregulation of e-cadherin and desmoglein by epidermal growth factor.
32-6100 was used in western blot to investigate the effect of EGF on the expression of E-cadherin and desmoglein and its mechanism.
|Chavez MG,Buhr CA,Petrie WK,Wandinger-Ness A,Kusewitt DF,Hudson LG||Dermatology research and practice (2012:null)||2012|
Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection.
32-6100 was used in immunohistochemistry to examine the contribution of the desmosomal compartment to gastroesophageal reflux disease.
|Wex T,Kuester D,Mönkemüller K,Stahr A,Fry LC,Kandulski A,Kropf S,Roessner A,Malfertheiner P||Human pathology (43:1745)||2012|
Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa.
32-6100 was used in immunohistochemistry to examine the role of desmosomal proteins in gastro-oesophageal reflux disease.
|Wex T,Mönkemüller K,Stahr A,Kuester D,Fry LC,Völkel S,Kandulski A,Roessner A,Malfertheiner P||Histopathology (60:405)||2012|
|Human||Not Cited||Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease.||Silberberg M,Charron AJ,Bacallao R,Wandinger-Ness A||American journal of physiology. Renal physiology (288:F1153)||2005|