Invitrogen

Desmoglein 2 Monoclonal Antibody (6D8)

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Desmoglein 2 Antibody in Western Blot (WB) — Western blot analysis of Desmoglein-2 was performed by loading 30 µg of HEK-293 (lane1), A549 (lane2), A-431 (lane3), and K562 (lane4) cell lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and Pierce™ Power Blotter System (22834). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Desmoglein-2 was detected at ~122 kDa using Desmoglein-2 Mouse Monoclonal Antibody (Product # 32-6100) at 1-2 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Desmoglein 2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — 2 Fig. Disruption of desmosome function leads to increased centrosome clustering. (A) Western blot analysis of indicated proteins in HaCaT, Vec, 14-3-3epsilon KO, and 14-3-3gamma KO cell lines. Blots for actin served as loading controls. (B, C) The indicated cell lines were stained with antibodies to the indicated proteins. Representative immunofluorescence images are shown (B) and a bar graph showing the mean fluorescence intensity of indicated proteins at the cell border (C). (D, E) Immunofluorescence images of mitotic cells stained with antibodies to PG (red) or DPI/II (red) and DAPI (blue). Arrows indicate mitotic cells. Representative images are shown (D) and a bar graph showing the mean fluorescence intensity of PG or DPI/II at the cell border (E). (F) Box and whisker plot showing cell stiffness of mitotic cells from the indicated cell lines as determined by AFM. The mean and standard deviation from three independent experiments is plotted, and P -valueswere obtained using Student's unpaired t -test (two-tailed). ** P < 0.01, *** P < 0.005. ns indicates not significant. Scale = 10 um.

Desmoglein 2 Antibody in Immunohistochemistry (IHC) — Figure 8 Immunohistochemistry of SgII and Dsg2 on paraffin sections of primary malignant melanomas and melanoma metastases. (A-C, J-L) Primary nodular malignant melanoma (NMM) with a tumor thickness of 0.8 mm (pT1a; no. 4 in the Table S3 ). (D-F, M-O) Primary NMM of 10 mm thickness (pT4a; no. 1). (G-I) Melanoma metastasis of the mamma (no. 8). (P-R) Cutaneous melanoma metastasis of the temple (no. 15). In the upper part of the figure (A-I), tumors were immunostained with two different SgII antisera (A, D, G: GTX116446 and B, E, H: LS-C39034), or, for control, with secondary goat anti-rabbit HRP-IgG (C, F, I). Clearly positive immunoreactions are seen in the cytoplasm of the melanoma cells with both SgII antisera in all tumors examined. In the lower part (J-R), tumors were reacted with antibodies against Dsg2 (rb5). Diffuse Dsg2-positive reactions are detected in the cytoplasm and/or at the surface of the melanoma cells (J, M, P: overviews; K, N, Q: higher magnifications). In addition, Dsg2 appears to be focally enhanced at the cell-cell contacts of the primary NMM of 10 mm thickness and the cutaneous metastasis (N, Q). Dsg2-positive structures serving as internal positive controls on each slide, i.e. sweat glands (L), basal epidermis (O) and hair follicles (R) are shown on the right hand side of each row. Bars: 50 um (L, Q), 200 um (P) or 100 um (all other micrographs).

Product Details

32-6100

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	Assay-dependent	View 4 publications 4 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	5-10 µg	-
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Bovine, Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	6D8
Immunogen	Human desmoglein-2.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533089

Target Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.

FEBS letters

Tilwani S,Gandhi K,Narayan S,Ainavarapu SRK,Dalal SN

Published figure using Desmoglein 2 monoclonal antibody (Product # 32-6100) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.

FEBS letters

Tilwani S,Gandhi K,Narayan S,Ainavarapu SRK,Dalal SN

Published figure using Desmoglein 2 monoclonal antibody (Product # 32-6100) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Leaky Gut as a Potential Culprit for the Paradoxical Dysglycemic Response to Gastric Bypass-Associated Ileal Microbiota.

Metabolites

Hankir MK,Seyfried F,Schellinger IN,Schlegel N,Arora T

32-6100 was used in Immunocytochemistry, Western Blot to study how Roux-en-Y gastric bypass (RYGB)-associated ileal microbiota can paradoxically impair host glycemic control when transferred to germ-free mice.

Mon Mar 08 00:00:00 EST 2021

Leaky Gut as a Potential Culprit for the Paradoxical Dysglycemic Response to Gastric Bypass-Associated Ileal Microbiota.

Metabolites

Hankir MK,Seyfried F,Schellinger IN,Schlegel N,Arora T

Mon Mar 08 00:00:00 EST 2021

Leaky Gut as a Potential Culprit for the Paradoxical Dysglycemic Response to Gastric Bypass-Associated Ileal Microbiota.

Metabolites

Hankir MK,Seyfried F,Schellinger IN,Schlegel N,Arora T

Mon Mar 08 00:00:00 EST 2021

Leaky Gut as a Potential Culprit for the Paradoxical Dysglycemic Response to Gastric Bypass-Associated Ileal Microbiota.

Metabolites

Hankir MK,Seyfried F,Schellinger IN,Schlegel N,Arora T

Mon Mar 08 00:00:00 EST 2021

The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system.

Cell and tissue research

Domke LM,Franke WW

32-6100 was used in Immunocytochemistry-immunoflourescence to show the LSMCs of the peritubular wall monolayers are SMCs sensu stricto which are laterally connected by a novel architectonic system of arrays of vertical AJs located in overlapping cell protrusions.

Fri Feb 01 00:00:00 EST 2019

A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis.

Angiogenesis

Ebert LM,Tan LY,Johan MZ,Min KK,Cockshell MP,Parham KA,Betterman KL,Szeto P,Boyle S,Silva L,Peng A,Zhang Y,Ruszkiewicz A,Zannettino AC,Gronthos S,Koblar S,Harvey NL,Lopez AF,Shackleton M,Bonder CS

326100 was used in flow cytometry and immunohistochemistry - paraffin section to study the function of desmoglein-2 and generate knockout mice

Sat Oct 01 00:00:00 EDT 2016

CUB domain-containing protein 1 and the epidermal growth factor receptor cooperate to induce cell detachment.

Breast cancer research : BCR

Law ME,Ferreira RB,Davis BJ,Higgins PJ,Kim JS,Castellano RK,Chen S,Luesch H,Law BK

326100 was used in western blot to study how CUB domain-containing protein1 contributes to metastasis and drug resistance

Fri Aug 05 00:00:00 EDT 2016

Desmoglein 2 promotes vasculogenic mimicry in melanoma and is associated with poor clinical outcome.

Oncotarget

Tan LY,Mintoff C,Johan MZ,Ebert BW,Fedele C,Zhang YF,Szeto P,Sheppard KE,McArthur GA,Foster-Smith E,Ruszkiewicz A,Brown MP,Bonder CS,Shackleton M,Ebert LM

32-6100 was used in Flow cytometry/Cell sorting to study the mechanisms that regulate vasculogenic mimicry.

Tue Jul 19 00:00:00 EDT 2016

Desmoglein 2 depletion leads to increased migration and upregulation of the chemoattractant secretoneurin in melanoma cells.

PloS one

Peitsch WK,Doerflinger Y,Fischer-Colbrie R,Huck V,Bauer AT,Utikal J,Goerdt S,Schneider SW

32-6100 was used in immunocytochemistry and western blot to determine the role of desmoglein 2 in melanoma cells

Tue Oct 21 00:00:00 EDT 2014

Desmoglein 2 depletion leads to increased migration and upregulation of the chemoattractant secretoneurin in melanoma cells.

PloS one

Peitsch WK,Doerflinger Y,Fischer-Colbrie R,Huck V,Bauer AT,Utikal J,Goerdt S,Schneider SW

32-6100 was used in immunocytochemistry and western blot to determine the role of desmoglein 2 in melanoma cells

Tue Oct 21 00:00:00 EDT 2014

Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection.

Human pathology

Wex T,Kuester D,Mönkemüller K,Stahr A,Fry LC,Kandulski A,Kropf S,Roessner A,Malfertheiner P

32-6100 was used in immunohistochemistry to examine the contribution of the desmosomal compartment to gastroesophageal reflux disease.

Mon Oct 01 00:00:00 EDT 2012

Differential downregulation of e-cadherin and desmoglein by epidermal growth factor.

Dermatology research and practice

Chavez MG,Buhr CA,Petrie WK,Wandinger-Ness A,Kusewitt DF,Hudson LG

32-6100 was used in western blot to investigate the effect of EGF on the expression of E-cadherin and desmoglein and its mechanism.

Thu Aug 23 00:00:00 EDT 2012

Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa.

Histopathology

Wex T,Mönkemüller K,Stahr A,Kuester D,Fry LC,Völkel S,Kandulski A,Roessner A,Malfertheiner P

32-6100 was used in immunohistochemistry to examine the role of desmosomal proteins in gastro-oesophageal reflux disease.

Wed Feb 01 00:00:00 EST 2012

Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease.

American journal of physiology. Renal physiology

Silberberg M,Charron AJ,Bacallao R,Wandinger-Ness A

Wed Jun 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: ARVC10; ARVD10; Cadherin family member 5; CMD1BB; Desmoglein-2; DSG-2; HDGC; MGC117034; MGC117036; MGC117037

Gene Aliases: CDHF5; DSG2; HDGC

UniProt ID: (Human) Q14126

Entrez Gene ID: (Human) 1829

Molecular Function: cadherin

Function(s)

desmosome organization Purkinje myocyte development cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules response to progesterone maternal process involved in female pregnancy bundle of His cell-Purkinje myocyte adhesion involved in cell communication regulation of heart rate by cardiac conduction regulation of ventricular cardiac muscle cell action potential

Process(es)

calcium ion binding cell adhesion molecule binding cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication

Desmoglein 2 Monoclonal Antibody (6D8)

Product Details

32-6100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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