Invitrogen

Desmoglein 2 Monoclonal Antibody (CSTEM28), eBioscience™

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Desmoglein 2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry of fixed and permeabilized 2102EP cells using 10 µg/mL Anti-Human Desmoglein 2 Purified followed by F (ab')2 Anti-Mouse IgG eFluor® 660. Nuclei are stained with DAPI.

Desmoglein 2 Antibody in Flow Cytometry (Flow) — Staining of a mixture of iPSC and the C2C12 cell line with 0.25 µg of Mouse IgG2b K Isotype Control Purified (Product # 14-4732-82) (blue histogram) or 0.25 µg of Anti-Human Desmoglein 2 Purified (purple histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82). Total viable cells were used for analysis.

Product Details

14-9159-82

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	10 µg/mL	-
Flow Cytometry (Flow)	0.5 µg/test	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG2b, kappa
Class	Monoclonal
Type	Antibody
Clone	CSTEM28
Conjugate	Unconjugated Alexa Fluor 488 PE
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2637283

Product Specific Information

This monoclonal antibody CSTEM28 reacts with human desmoglein 2 (DSG2), a type 1 transmembrane protein belonging to the cadherin family. DSG2 is expressed by epithelial cells, cardiomyocytes, endothelial progenitors, and CD34+CD45dim hematopoietic stem and progenitor cells. This adhesion molecule forms cell-cell adhesion complexes called desmosomes that provide tensile strength to tissues that experience mechanical stress. Other than its role in desmosome formation, DSG2 plays a role in barrier integrity, adhesion, migration, cell apoptosis, proliferation, vasculogenic mimicry, and intracellular signaling. DSG2-expressing endothelial progenitor cells are pro-angiogenic, suggesting a role in vasculature formation. On hematopoietic stem and progenitor cells, DSG2 expression is highest on CD38-CD90+ multi-potent cells. DSG2 expression is progressively decreased as a function of differentiation, with DSG2 expression absent on mature lymphocytes. DSG2 also serves as a receptor for adenoviruses and can be shed by mucosal barriers in the intestine.

This CSTEM28 antibody has been tested by flow cytometric analysis of the 2102ep cell line. This has also been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Combination of immunogenic oncolytic adenovirus ONCOS-102 with anti-PD-1 pembrolizumab exhibits synergistic antitumor effect in humanized A2058 melanoma huNOG mouse model.

Oncoimmunology

Kuryk L,Møller AW,Jaderberg M

Published figure using Desmoglein 2 monoclonal antibody (Product # 14-9159-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Biology

Kutova OM,Sencha LM,Pospelov AD,Dobrynina OE,Brilkina AA,Cherkasova EI,Balalaeva IV

Published figure using Desmoglein 2 monoclonal antibody (Product # 14-9159-82) in Flow Cytometry

Fri Dec 04 00:00:00 EST 2020

Chimeric oncolytic Ad5/3 virus replicates and lyses ovarian cancer cells through desmoglein-2 cell entry receptor.

Journal of medical virology

Kuryk L,Møller AW

Published figure using Desmoglein 2 monoclonal antibody (Product # 14-9159-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: ARVC10; ARVD10; Cadherin family member 5; CMD1BB; Desmoglein-2; DSG-2; HDGC; MGC117034; MGC117036; MGC117037

Gene Aliases: CDHF5; DSG2; HDGC

UniProt ID: (Human) Q14126

Entrez Gene ID: (Human) 1829

Molecular Function: cadherin

Function(s)

desmosome organization Purkinje myocyte development cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules response to progesterone maternal process involved in female pregnancy bundle of His cell-Purkinje myocyte adhesion involved in cell communication regulation of heart rate by cardiac conduction regulation of ventricular cardiac muscle cell action potential

Process(es)

calcium ion binding cell adhesion molecule binding cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication

Desmoglein 2 Monoclonal Antibody (CSTEM28), eBioscience™

Product Details

14-9159-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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