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|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Desmoglein 3 recombinant protein.|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes.
MA5-16025 was used in immunocytochemistry and western blot to study the role of desmogleins 2 and 3 in the regulation of keratinocyte adhesion
|Hartlieb E,Rötzer V,Radeva M,Spindler V,Waschke J||The Journal of biological chemistry (289:17043)||2014|
Adducin is required for desmosomal cohesion in keratinocytes.
MA5-16025 was used in immunocytochemistry to study the role of actin-binding protein adducin in desmosomal cohesion in keratinocytes
|Rötzer V,Breit A,Waschke J,Spindler V||The Journal of biological chemistry (289:14925)||2014|
De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells.
MA5-16025 was used in immunohistochemistry to study the role of human eccrine sweat gland cells during epidermal regeneration
|Pontiggia L,Biedermann T,Böttcher-Haberzeth S,Oliveira C,Braziulis E,Klar AS,Meuli-Simmen C,Meuli M,Reichmann E||The Journal of investigative dermatology (134:1735)||2014|
Altered expression and localization of desmoglein 3 in esophageal squamous cell carcinoma.
MA5-16025 was used in immunohistochemistry - paraffin section to examine desmoglein 3 expression and subcellular localization in esophageal squamous cell carcinoma.
|Fang WK,Chen B,Xu XE,Liao LD,Wu ZY,Wu JY,Shen J,Xu LY,Li EM||Acta histochemica (116:803)||2014|
Desmosome assembly and disassembly are membrane raft-dependent.
MA5-16025 was used in western blot to investigate the role of membrane rafts during desmosome assembly and disassembly and cell adhesion
|Stahley SN,Saito M,Faundez V,Koval M,Mattheyses AL,Kowalczyk AP||PloS one (9:null)||2014|
130 kDa pemphigus vulgaris antigen; 130-kD pemphigus vulgaris antigen; cadherin family member 6; CDHF6; desmoglein-3; pemphigus vulgaris antigen; PVA
CDHF6; DSG3; PVA