Samples were separated by SDS-PAGE (gradient gel 10-20%). Bands were transferred to a PVDF membrane which was incubated with the primary antibody at the recommended working concentration. AP-conjugated GAM secondary antibodies were used at a 1:3000 dilution for detection and signal was developed with AP development kit.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant SMAC, TIM22 proteins|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Inhibitors of apoptosis proteins (IAP's) block cell death by caspase inhibition. X linked inhibitor of apoptosis (XIAP) is a member of the IAP family. Smac/Diablo is expressed in a variety of tissues and has been identified as a negative regulator of XIAP. Smac/Diablo is a mitochondrial protein that enters the cytosol when cells undergo apoptosis, and moderates the caspase inhibition of IAPs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.