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Dicer Monoclonal Antibody (N167/7)

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)

FIGURE: 1 / 1

Dicer Antibody (39817) in WB

Dicer antibody (mAb) tested by Western blot. Cytoplasmic extract of mouse ES cells (40 µg per lane) probed with Dicer antibody (2 µg/ml).

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Product Details

39817

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N167/7

Immunogen

This Dicer antibody was raised against a recombinant protein corresponding to amino acids 1638-1899 of mouse Dicer.

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Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

70mM tris, pH 8, with 0.07mM EDTA, 105mM NaCl, 31mM glycine, 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2793353

Target Information

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DCR; DCR-1; dicer 1 ribonuclease III; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; double-strand-specific ribonuclease mDCR-1; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; helicase-moi

Gene Aliases: 1110006F08Rik; D12Ertd7e; DCR1; DICER; DICER1; Dicer1e; HERNA; K12H4.8-LIKE; KIAA0928; mKIAA0928; MNG1; RMSE2

UniProt ID: (Human) Q9UPY3

Entrez Gene ID: (Human) 23405, (Mouse) 192119

Function(s)

negative regulation of transcription from RNA polymerase II promoter meiotic spindle organization angiogenesis regulation of protein phosphorylation hair follicle development apoptotic DNA fragmentation RNA processing cell proliferation regulation of Notch signaling pathway post-embryonic development zygote asymmetric cell division regulation of gene expression negative regulation of Schwann cell proliferation positive regulation of gene expression regulation of muscle cell apoptotic process positive regulation of Schwann cell differentiation myoblast differentiation involved in skeletal muscle regeneration rRNA catabolic process stem cell population maintenance spinal cord motor neuron differentiation nerve development olfactory bulb interneuron differentiation cerebral cortex development lung development embryonic limb morphogenesis production of siRNA involved in RNA interference targeting of mRNA for destruction involved in RNA interference gene silencing by RNA pre-miRNA processing hair follicle morphogenesis regulation of myelination positive regulation of myelination peripheral nervous system myelin formation conversion of ds siRNA to ss siRNA involved in RNA interference siRNA loading onto RISC involved in RNA interference embryonic hindlimb morphogenesis production of miRNAs involved in gene silencing by miRNA multicellular organism growth miRNA loading onto RISC involved in gene silencing by miRNA conversion of ds siRNA to ss siRNA regulation of odontogenesis of dentin-containing tooth regulation of viral genome replication regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter mRNA stabilization spleen development digestive tract development reproductive structure development regulation of oligodendrocyte differentiation epidermis morphogenesis branching morphogenesis of an epithelial tube neuron projection morphogenesis anatomical structure development regulation of neurogenesis cartilage development spindle assembly regulation of RNA metabolic process defense response to virus regulation of cell cycle cardiac muscle cell development inner ear receptor cell development negative regulation of glial cell proliferation intestinal epithelial cell development cardiac neural crest cell development involved in outflow tract morphogenesis ganglion development regulation of enamel mineralization hair follicle cell proliferation RNA phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic regulation of miRNA metabolic process positive regulation of miRNA metabolic process

Process(es)

double-stranded RNA binding helicase activity endoribonuclease activity ribonuclease III activity deoxyribonuclease I activity protein binding ATP binding protein domain specific binding siRNA binding metal ion binding pre-miRNA binding nucleotide binding nucleic acid binding RNA binding nuclease activity endonuclease activity hydrolase activity endoribonuclease activity, producing 5'-phosphomonoesters miRNA binding