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Dicer Monoclonal Antibody (N167/7)

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Dicer Antibody in Western Blot (WB) — Dicer antibody (mAb) tested by Western blot. Cytoplasmic extract of mouse ES cells (40 µg per lane) probed with Dicer antibody (2 µg/ml).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Product Specifications

Species

Human, Mouse

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N167/7

Immunogen

This Dicer antibody was raised against a recombinant protein corresponding to amino acids 1638-1899 of mouse Dicer.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

70mM tris, pH 8, with 0.07mM EDTA, 105mM NaCl, 31mM glycine, 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2793353

Target Information

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DCR; DCR-1; dicer 1 ribonuclease III; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; double-strand-specific ribonuclease mDCR-1; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; helicase-moi

Gene Aliases: 1110006F08Rik; D12Ertd7e; DCR1; DICER; DICER1; Dicer1e; HERNA; K12H4.8-LIKE; KIAA0928; mKIAA0928; MNG1; RMSE2

UniProt ID: (Human) Q9UPY3

Entrez Gene ID: (Human) 23405, (Mouse) 192119

Molecular Function: DNA binding protein endodeoxyribonuclease nuclease

negative regulation of transcription from RNA polymerase II promoter apoptotic DNA fragmentation negative regulation of Schwann cell proliferation positive regulation of Schwann cell differentiation nerve development production of siRNA involved in RNA interference targeting of mRNA for destruction involved in RNA interference gene silencing by RNA pre-miRNA processing positive regulation of myelination peripheral nervous system myelin formation conversion of ds siRNA to ss siRNA involved in RNA interference siRNA loading onto RISC involved in RNA interference production of miRNAs involved in gene silencing by miRNA miRNA loading onto RISC involved in gene silencing by miRNA conversion of ds siRNA to ss siRNA neuron projection morphogenesis RNA phosphodiester bond hydrolysis, endonucleolytic meiotic spindle organization angiogenesis regulation of protein phosphorylation hair follicle development RNA processing cell proliferation regulation of Notch signaling pathway post-embryonic development zygote asymmetric cell division regulation of gene expression positive regulation of gene expression regulation of muscle cell apoptotic process myoblast differentiation involved in skeletal muscle regeneration rRNA catabolic process stem cell population maintenance spinal cord motor neuron differentiation olfactory bulb interneuron differentiation cerebral cortex development lung development embryonic limb morphogenesis hair follicle morphogenesis regulation of myelination embryonic hindlimb morphogenesis multicellular organism growth regulation of odontogenesis of dentin-containing tooth regulation of viral genome replication regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter mRNA stabilization spleen development digestive tract development reproductive structure development regulation of oligodendrocyte differentiation epidermis morphogenesis branching morphogenesis of an epithelial tube anatomical structure development regulation of neurogenesis cartilage development spindle assembly regulation of RNA metabolic process defense response to virus regulation of cell cycle cardiac muscle cell development inner ear receptor cell development negative regulation of glial cell proliferation intestinal epithelial cell development cardiac neural crest cell development involved in outflow tract morphogenesis ganglion development regulation of enamel mineralization hair follicle cell proliferation RNA phosphodiester bond hydrolysis regulation of miRNA metabolic process positive regulation of miRNA metabolic process

Process(es)

double-stranded RNA binding helicase activity endoribonuclease activity ribonuclease III activity deoxyribonuclease I activity protein binding ATP binding protein domain specific binding siRNA binding metal ion binding pre-miRNA binding nucleotide binding nucleic acid binding RNA binding nuclease activity endonuclease activity hydrolase activity endoribonuclease activity, producing 5'-phosphomonoesters miRNA binding