Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Digoxigenin (DIG), a steroid, is a derivative of the flowers and leaves of plants, i.e., Digitalis sp. Due to the high immunigenicity of this small molecule it is highly preferred in many molecular biology applications. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications. The digoxigenin markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes for visualization and detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C23H34O5; DIG; Digitalis