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Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Dihydropyridine Receptor alpha-1 (MA3-920 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
|Tested species reactivity||Guinea pig, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rabbit, Rat, Fish, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified rabbit muscle T-tubule dihydropyridine receptor.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-920 detects 1,4-dihydropyridine (DHP) receptor alpha-1 subunit in human, rat, mouse, guinea pig and rabbit skeletal muscle. The DHP Receptor alpha-1 protein is also known as CACNA1S or Cav1.1 alpha-1 subunit.
MA3-920 has been successfully used in Western blot, FACS, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing the DHP receptor in rat skeletal muscle extracts. Immunohistochemical staining of DHP receptor in rabbit skeletal muscle with MA3-920 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines. This product can be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.
The MA3-920 antigen is purified rabbit muscle T-tubule DHP receptor.
Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.
The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Downregulation of connexin43 by microRNA-130a in cardiomyocytes results in cardiac arrhythmias.
MA3-920 was used in western blot to study the role of connexin43 downregulation in the mechanism by which overexpression of miRNA-130a in cardiomyocytes induces cardiac arrhythmia
|Osbourne A,Calway T,Broman M,McSharry S,Earley J,Kim GH||Journal of molecular and cellular cardiology (74:53)||2014|
Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.
MA3-920 was used in western blot to study the significance for muscular dystrophy pathogenesis of the roles played by dysferlin and myoferlin in skeletal muscle glycerol sensitivity and transverse tubule formation
|Demonbreun AR,Rossi AE,Alvarez MG,Swanson KE,Deveaux HK,Earley JU,Hadhazy M,Vohra R,Walter GA,Pytel P,McNally EM||The American journal of pathology (184:248)||2014|
Effects of SEA0400 on arrhythmogenicity in a Langendorff-perfused 1-month myocardial infarction rabbit model.
MA3-920 was used in western blot to study arrythomogenicity in a Langendorff-perfused rabbit heart model of myocardial infarction and the effects of a Na(+)/Ca(2+) exchanger inhibitor
|Chou CC,Chang PC,Wen MS,Lee HL,Chu Y,Baba A,Matsuda T,Yeh SJ,Wu D||Pacing and clinical electrophysiology : PACE (36:596)||2013|
Myotubularin and PtdIns3P remodel the sarcoplasmic reticulum in muscle in vivo.
MA3-920 was used in western blot to study the role of the phosphoinositide phosphatase myotublarin in the remodeling of skeletal muscle sarcoplasmic reticulum
|Amoasii L,Hnia K,Chicanne G,Brech A,Cowling BS,Müller MM,Schwab Y,Koebel P,Ferry A,Payrastre B,Laporte J||Journal of cell science (126:1806)||2013|
Three-dimensional localization of the α and β subunits and of the II-III loop in the skeletal muscle L-type Ca2+ channel.
mA3-920 was used in western blot to study the 3-dimensional structure of the skeletal muscle L-type Ca(2+) channel and the localization of the alpha and beta subunits and the II-III loop via electron microscopy
|Szpyt J,Lorenzon N,Perez CF,Norris E,Allen PD,Beam KG,Samsó M||The Journal of biological chemistry (287:43853)||2012|
Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?
MA3-920 was used in western blot to investigate the abnormalities of calcium handling proteins in skeletal and heart muscle during heart failure
|Middlekauff HR,Vigna C,Verity MA,Fonarow GC,Horwich TB,Hamilton MA,Shieh P,Tupling AR||Journal of cardiac failure (18:724)||2012|
Muscle weakness in myotonic dystrophy associated with misregulated splicing and altered gating of Ca(V)1.1 calcium channel.
MA3-920 was used in western blot to investigate the association between the changes in Ca(V)1.1 calcium channel and muscle weakness in myotonic dystrophy
|Tang ZZ,Yarotskyy V,Wei L,Sobczak K,Nakamori M,Eichinger K,Moxley RT,Dirksen RT,Thornton CA||Human molecular genetics (21:1312)||2012|
The calcium channel α2/δ1 subunit interacts with ATP5b in the plasma membrane of developing muscle cells.
MA3-920 was used in western blot to study the binding dynamics of ATP5b subunits
|García J||American journal of physiology. Cell physiology (301:C44)||2011|
Identification of novel ryanodine receptor 1 (RyR1) protein interaction with calcium homeostasis endoplasmic reticulum protein (CHERP).
MA3-920 was used in western blot to identify interacting partners of the skeletal muscle receptor, ryanodine receptor 1
|Ryan T,Sharma P,Ignatchenko A,MacLennan DH,Kislinger T,Gramolini AO||The Journal of biological chemistry (286:17060)||2011|
Aerobic exercise training improves skeletal muscle function and Ca2+ handling-related protein expression in sympathetic hyperactivity-induced heart failure.
MA3-920 was used in western blot to investigate the effect of aerobic exercise on skeletal muscle function and calcium-related protein expression
|Bueno CR,Ferreira JC,Pereira MG,Bacurau AV,Brum PC||Journal of applied physiology (Bethesda, Md. : 1985) (109:702)||2010|
Initiating treadmill training in late middle age offers modest adaptations in Ca2+ handling but enhances oxidative damage in senescent rat skeletal muscle.
MA3-920 was used in western blot to identify the relationship between calcium handling and muscle senescence during late middle-age training
|Thomas MM,Vigna C,Betik AC,Tupling AR,Hepple RT||American journal of physiology. Regulatory, integrative and comparative physiology (298:R1269)||2010|
Aerobic exercise training improves Ca2+ handling and redox status of skeletal muscle in mice.
MA3-920 was used in western blot to investigate the effect of aerobic exercise on calcium signal and redox state of skeletal muscle
|Ferreira JC,Bacurau AV,Bueno CR,Cunha TC,Tanaka LY,Jardim MA,Ramires PR,Brum PC||Experimental biology and medicine (Maywood, N.J.) (235:497)||2010|
Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle.
MA3-920 was used in western blot to investigate the expression of sarcalumenin and related calcium-regulatory proteins in rodent skeletal muscle during aging
|O'Connell K,Gannon J,Doran P,Ohlendieck K||Experimental gerontology (43:958)||2008|
Exercise-induced regulation of phospholemman (FXYD1) in rat skeletal muscle: implications for Na+/K+-ATPase activity.
MA3-920 was used in western blot to study the effect of exercise on phospholemman and its mechanism
|Rasmussen MK,Kristensen M,Juel C||Acta physiologica (Oxford, England) (194:67)||2008|
A Ca2+-binding domain in RyR1 that interacts with the calmodulin binding site and modulates channel activity.
MA3-920 was used in western blot to study the interaction of a fragment of RyR1 (R4064-C4210) with Cav1.1
|Xiong L,Zhang JZ,He R,Hamilton SL||Biophysical journal (90:173)||2006|
Regulation of dihydropyridine receptor gene expression in mouse skeletal muscles by stretch and disuse.
MA3-920 was used in western blot to study the change of DHPR's gene expression in mouse skeletal muscles during physiological adaptations to disuse
|Radzyukevich TL,Heiny JA||American journal of physiology. Cell physiology (287:C1445)||2004|
Developmental induction of DHPR alpha 1s and RYR1 gene expression does not require neural or mechanical signals.
MA3-920 was used in western blot to compare the expression of DHPR/alpha1s and RyR1 in the diaphragm and hindlimb skeletal muscles of neonatal mice
|Radzyukevich TL,Cougnon MH,Moseley AE,Heiny JA||Journal of muscle research and cell motility (25:87)||2004|
Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle.
MA3-920 was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia
|Glover L,Heffron JJ,Ohlendieck K||Journal of applied physiology (Bethesda, Md. : 1985) (96:11)||2004|
Suramin interacts with the calmodulin binding site on the ryanodine receptor, RYR1.
MA3-920 was used in western blot to investigate the interaction between suramin and ryanodine receptor RYR1.
|Papineni RV,O'Connell KM,Zhang H,Dirksen RT,Hamilton SL||The Journal of biological chemistry (277:49167)||2002|
Na,K-ATPase alpha- and beta-isoform expression in developing skeletal muscles: alpha(2) correlates with t-tubule formation.
MA3-920 was used in western blot to investigate the expression of sodium/potassium ATPase alpha- and beta- isoforms in skeletal muscles during development
|Cougnon MH,Moseley AE,Radzyukevich TL,Lingrel JB,Heiny JA||Pflu¿gers Archiv : European journal of physiology (445:123)||2002|
Ca2+-induced Ca2+ release supports the relay mode of activity in thalamocortical cells.
MA3-920 was used in western blot to investigate the mechanism for the calcium-induced calcium release in thalamocortical cells
|Budde T,Sieg F,Braunewell KH,Gundelfinger ED,Pape HC||Neuron (26:483)||2000|
A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function.
MA3-920 was used in western blot to characterize a transgenic myogenic cell line without functioning skeletal muscle ryanodine receptor.
|Moore RA,Nguyen H,Galceran J,Pessah IN,Allen PD||The Journal of cell biology (140:843)||1998|
Dyspedic mouse skeletal muscle expresses major elements of the triadic junction but lacks detectable ryanodine receptor protein and function.
MA3-920 was used in western blot to demonstrate that the proteins expressed in dyspedic skeletal muscle in the absence of Ry1R are critical for ryanodine receptor function
|Buck ED,Nguyen HT,Pessah IN,Allen PD||The Journal of biological chemistry (272:7360)||1997|
EHD1 mediates vesicle trafficking required for normal muscle growth and transverse tubule development.
MA3-920 was used in immunohistochemistry to study the role of vesicle trafficking and cytoskeletal reorganization in the mechanism by which EHD1 mediates normal muscle development
|Posey AD,Swanson KE,Alvarez MG,Krishnan S,Earley JU,Band H,Pytel P,McNally EM,Demonbreun AR||Developmental biology (387:179)||2014|
Gene therapy prolongs survival and restores function in murine and canine models of myotubular myopathy.
MA3-920 was used in immunohistochemistry to study the beneficial effects of MTM1 gene therapy in mouse and dog models of myotubular myopathy
|Childers MK,Joubert R,Poulard K,Moal C,Grange RW,Doering JA,Lawlor MW,Rider BE,Jamet T,Danièle N,Martin S,Rivière C,Soker T,Hammer C,Van Wittenberghe L,Lockard M,Guan X,Goddard M,Mitchell E,Barber J,Williams JK,Mack DL,Furth ME,Vignaud A,Masurier C,Mavilio F,Moullier P,Beggs AH,Buj-Bello A||Science translational medicine (6:null)||2014|
Lack of myotubularin (MTM1) leads to muscle hypotrophy through unbalanced regulation of the autophagy and ubiquitin-proteasome pathways.
MA3-920 was used in immunohistochemistry to study the role of the autophagy and ubiquitin-proteasome pathways in the mechanism by which lack of myotubularin results in muscle hypotrophy
|Al-Qusairi L,Prokic I,Amoasii L,Kretz C,Messaddeq N,Mandel JL,Laporte J||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (27:3384)||2013|
Domain cooperativity in the β1a subunit is essential for dihydropyridine receptor voltage sensing in skeletal muscle.
MA3-920 was used in immunohistochemistry to study the role of domain cooperativity within the beta1a subunit in the voltage-sensing ability of the skeletal muscle dihydropyridine receptor
|Dayal A,Bhat V,Franzini-Armstrong C,Grabner M||Proceedings of the National Academy of Sciences of the United States of America (110:7488)||2013|
Defects in amphiphysin 2 (BIN1) and triads in several forms of centronuclear myopathies.
MA3-920 was used in immunohistochemistry to investigate the effect of BIN1 dysfunction on the pathogenesis of centronuclear myopathies
|Toussaint A,Cowling BS,Hnia K,Mohr M,Oldfors A,Schwab Y,Yis U,Maisonobe T,Stojkovic T,Wallgren-Pettersson C,Laugel V,Echaniz-Laguna A,Mandel JL,Nishino I,Laporte J||Acta neuropathologica (121:253)||2011|
Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy.
MA3-920 was used in immunohistochemistry to investigate the immunohistochemical features of hexagonally cross-linked tubular arrays myopathy
|Di Blasi C,Blasevich F,Bellafiore E,Mottarelli E,Gibertini S,Zanotti S,Saredi S,Mantegazza R,Morandi L,Mora M||Neuromuscular disorders : NMD (20:326)||2010|
T-tubule disorganization and defective excitation-contraction coupling in muscle fibers lacking myotubularin lipid phosphatase.
MA3-920 was used in immunohistochemistry to investigate the mechanisms for the loss of muscle function in X-linked myotubular myopathy.
|Al-Qusairi L,Weiss N,Toussaint A,Berbey C,Messaddeq N,Kretz C,Sanoudou D,Beggs AH,Allard B,Mandel JL,Laporte J,Jacquemond V,Buj-Bello A||Proceedings of the National Academy of Sciences of the United States of America (106:18763)||2009|
Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease.
MA3-920 was used in immunohistochemistry to study zebrafish relatively relaxed mutants carrying a ryanodine receptor defect as a model of multi-minicore disease
|Hirata H,Watanabe T,Hatakeyama J,Sprague SM,Saint-Amant L,Nagashima A,Cui WW,Zhou W,Kuwada JY||Development (Cambridge, England) (134:2771)||2007|
Restricted distribution of mRNAs encoding a sarcoplasmic reticulum or transverse tubule protein in skeletal myofibers.
MA3-920 was used in immunohistochemistry to investigate the distribution of CSQ and DHPR proteins and corresponding mRNAs during myogenic development
|Nissinen M,Kaisto T,Salmela P,Peltonen J,Metsikkö K||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (53:217)||2005|
Neuregulin receptor ErbB2 localization at T-tubule in cardiac and skeletal muscle.
MA3-920 was used in immunohistochemistry to locate neuregulin receptor ErbB2 at T-tubule in cardiac and skeletal muscle
|Ueda H,Oikawa A,Nakamura A,Terasawa F,Kawagishi K,Moriizumi T||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (53:87)||2005|
Dysferlin stabilizes stress-induced Ca2+ signaling in the transverse tubule membrane.
MA3-920 was used in immunocytochemistry to study the role of dysferlin in transverse tubule membrane stress-induced Ca(2+) signaling
|Kerr JP,Ziman AP,Mueller AL,Muriel JM,Kleinhans-Welte E,Gumerson JD,Vogel SS,Ward CW,Roche JA,Bloch RJ||Proceedings of the National Academy of Sciences of the United States of America (110:20831)||2013|
Homer proteins and InsP(3) receptors co-localise in the longitudinal sarcoplasmic reticulum of skeletal muscle fibres.
MA3-920 was used in immunocytochemistry to study the colocalization of Homer proteins and inositol trisphosphate receptors in the sarcoplasmic reticulum of skeletal muscle
|Salanova M,Priori G,Barone V,Intravaia E,Flucher B,Ciruela F,McIlhinney RA,Parys JB,Mikoshiba K,Sorrentino V||Cell calcium (32:193)||2002|
Sarcolemmal organization in skeletal muscle lacking desmin: evidence for cytokeratins associated with the membrane skeleton at costameres.
MA3-920 was used in immunocytochemistry to study the organization of the sarcolemma and cytokeratins in mouse muscle lacking desmin
|O'Neill A,Williams MW,Resneck WG,Milner DJ,Capetanaki Y,Bloch RJ||Molecular biology of the cell (13:2347)||2002|
Agonists cause endocytosis of nicotinic acetylcholine receptors on cultured myotubes.
MA3-920 was used in immunocytochemistry to study the role of agonists in the endocytosis of nicotinic acetylcholine receptors on cultured myotubes
|St John PA,Gordon H||Journal of neurobiology (49:212)||2001|
Cloning of a calcium channel alpha1 subunit from the reef-building coral, Stylophora pistillata.
MA3-920 was used in immunocytochemistry to determine the localization of the coral DHP/alpha1 calcium channel
|Zoccola D,Tambutté E,Sénégas-Balas F,Michiels JF,Failla JP,Jaubert J,Allemand D||Gene (227:157)||1999|
Branching points of renal resistance arteries are enriched in L-type calcium channels and initiate vasoconstriction.
MA3-920 was used in immunocytochemistry to investigate the distribution of L-type calcium channels near the branching points of renal resistance arteries and the relationship with smooth muscle cell function
|Goligorsky MS,Colflesh D,Gordienko D,Moore LC||The American journal of physiology (268:F251)||1995|
CACNA1S; Calcium Channel; calcium channel, L type, alpha 1 polypeptide, isoform 3 (skeletal muscle, hypokalemic periodic paralysis); calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; calcium channel, voltage-dependent, L type, alpha 1S subunit; Cav1.1 alpha-1; DHPR alpha1s; dihydropyridine receptor; dihydropyridine receptor alpha 1 subunit; dihydropyridine receptor alpha 1S; dihydropyridine-sensitive L-type calcium channel alpha-1 subunit; muscle dysgenesis; ROB1; voltage-dependent L-type calcium channel subunit alpha-1S; voltage-gated calcium channel subunit alpha Cav1.1
AW493108; CACH1; CACN1; CACNA1S; CACNL1A3; Cav1.1; CCHL1A3; fmd; HOKPP; HOKPP1; hypoPP; mdg; MHS5; sj; TTPP1