|Tested species reactivity||Donkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||donkey IgG whole molecule|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml BSA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:5000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:5000|
|Western Blot (WB)||1:20,000-1:400,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28591 detects Donkey IgG from donkey samples.
PA1-28591 has been successfully used in ELISA, immunohistochemistry (frozen tissue), Western blot, immunohistochemistry (paraffin tissue) applications.
The PA1-28591 immunogen is: donkey IgG whole molecule
Thermo Scientific Anti-Equine secondary antibodies are affinity-purified antibodies with well-characterized specificity for equine (horse) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.