|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Conjugate||Alexa Fluor® 350|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Boundary cap neural crest stem cells homotopically implanted to the injured dorsal root transitional zone give rise to different types of neurons and glia in adult rodents.
A10035 was used in immunohistochemistry - frozen section to study the fate of mouse boundary cap neural crest stem cells implanted in the dorsal root transitional zone
|Trolle C,Konig N,Abrahamsson N,Vasylovska S,Kozlova EN||BMC neuroscience (15:null)||2014|
|Not Applicable||Not Cited||The herpes simplex virus 1 Us11 protein inhibits autophagy through its interaction with the protein kinase PKR.||Lussignol M,Queval C,Bernet-Camard MF,Cotte-Laffitte J,Beau I,Codogno P,Esclatine A||Journal of virology (87:859)||2013|
|Not Applicable||Not Cited||Cysteine proteases bleomycin hydrolase and cathepsin Z mediate N-terminal proteolysis and toxicity of mutant huntingtin.||Ratovitski T,Chighladze E,Waldron E,Hirschhorn RR,Ross CA||The Journal of biological chemistry (286:12578)||2011|