Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and HeLa (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.5 µg/ml) and detected using Donkey anti-Mouse IgG Secondary Antibody, Alexa Fluor 680 (Product # A10038) at dilutions 0.04 µg/ml (Fig. 1), 0.1 µg/ml (Fig. 2) and 0.2 µg/ml (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Conjugate||Alexa Fluor® 680|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:500|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Western Blot (WB)||0.04-0.2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 6 publications below|
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities. This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease.||Sutton LM,Sanders SS,Butland SL,Singaraja RR,Franciosi S,Southwell AL,Doty CN,Schmidt ME,Mui KK,Kovalik V,Young FB,Zhang W,Hayden MR||Human molecular genetics (22:452)||2013|
|Not Applicable||Not Cited||A steep phosphoinositide bis-phosphate gradient forms during fungal filamentous growth.||Vernay A,Schaub S,Guillas I,Bassilana M,Arkowitz RA||The Journal of cell biology (198:711)||2012|
|Not Applicable||Not Cited||Deletion of G¿Z protein protects against diet-induced glucose intolerance via expansion of ß-cell mass.||Kimple ME,Moss JB,Brar HK,Rosa TC,Truchan NA,Pasker RL,Newgard CB,Casey PJ||The Journal of biological chemistry (287:20344)||2012|
|Not Applicable||Not Cited||Inactivation of the mitochondrial carrier SLC25A25 (ATP-Mg2+/Pi transporter) reduces physical endurance and metabolic efficiency in mice.||Anunciado-Koza RP,Zhang J,Ukropec J,Bajpeyi S,Koza RA,Rogers RC,Cefalu WT,Mynatt RL,Kozak LP||The Journal of biological chemistry (286:11659)||2011|
|Not Applicable||Not Cited||Reconstitution of the ancestral glycoprotein of human endogenous retrovirus k and modulation of its functional activity by truncation of the cytoplasmic domain.||Hanke K,Kramer P,Seeher S,Beimforde N,Kurth R,Bannert N||Journal of virology (83:12790)||2009|
|Not Applicable||Not Cited||Identification of thioredoxin-2 as a regulator of the mitochondrial permeability transition.||He M,Cai J,Go YM,Johnson JM,Martin WD,Hansen JM,Jones DP||Toxicological sciences : an official journal of the Society of Toxicology (105:44)||2008|