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Simultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes. Red: sog labeled using aminoallyl UTP (Cat. No. A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Cat. No. A20006, A20106). Green: ind labeled with DNP, followed by rabbit anti-dinitrophenyl-KLH IgG antibody (Cat. No. A6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Cat. No. Z25305). Blue: en labeled with biotin and detected with HRP-streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit #39, T30952). Yellow: wg labeled with digoxigenin and detected with sheep anti-digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti-Sheep IgG antibody (Cat. No. A11016). Magenta: msh labeled with fluorescein and detected with mouse anti-fluorescein/Oregon Green® IgG2a antibody (Cat. No. A6421) and Alexa Fluor® 488 Goat Anti-Mouse IgG antibody (Cat. No. A11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.
|Tested species reactivity||Sheep|
|Host / Isotype||Donkey / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse, rabbit, bovine and human sera and human IgG|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Ovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.