|Tested species reactivity||Sheep|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 568|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse, rabbit, bovine and human sera and human IgG|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 6 publications below|
Anti-Ovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||A Rab11a-enriched subapical membrane compartment regulates a cytoskeleton-dependent transcytotic pathway in secretory epithelial cells of the lacrimal gland.||Xu S,Edman M,Kothawala MS,Sun G,Chiang L,Mircheff A,Zhu L,Okamoto C,Hamm-Alvarez S||Journal of cell science (124:3503)||2011|
|Not Applicable||Not Cited||Intramembranous valine linked to schizophrenia is required for neuregulin 1 regulation of the morphological development of cortical neurons.||Chen Y,Hancock ML,Role LW,Talmage DA||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:9199)||2010|
|Not Applicable||Not Cited||Extrasynaptic release of GABA by retinal dopaminergic neurons.||Hirasawa H,Puopolo M,Raviola E||Journal of neurophysiology (102:146)||2009|
|Not Applicable||Not Cited||Three weeks of postweaning exercise in DIO rats produces prolonged increases in central leptin sensitivity and signaling.||Patterson CM,Bouret SG,Dunn-Meynell AA,Levin BE||American journal of physiology. Regulatory, integrative and comparative physiology (296:R537)||2009|
|Not Applicable||Not Cited||Elevated prolactin redirects secretory vesicle traffic in rabbit lacrimal acinar cells.||Wang Y,Chiu CT,Nakamura T,Walker AM,Petridou B,Trousdale MD,Hamm-Alvarez SF,Schechter JE,Mircheff AK||American journal of physiology. Endocrinology and metabolism (292:E1122)||2007|
|Not Applicable||Not Cited||The Nef protein of human immunodeficiency virus is a broad-spectrum modulator of chemokine receptor cell surface levels that acts independently of classical motifs for receptor endocytosis and Galphai signaling.||Michel N,Ganter K,Venzke S,Bitzegeio J,Fackler OT,Keppler OT||Molecular biology of the cell (17:3578)||2006|