|Tested species reactivity||Sheep|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 680|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse, rabbit, bovine and human sera and human IgG|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Western Blot (WB)||0.04-0.2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 10 publications below|
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
Anti-Ovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Docking of PRAK/MK5 to the atypical MAPKs ERK3 and ERK4 defines a novel MAPK interaction motif.||Aberg E,Torgersen KM,Johansen B,Keyse SM,Perander M,Seternes OM||The Journal of biological chemistry (284:19392)||2009|
|Not Applicable||Not Cited||Aftiphilin and gamma-synergin are required for secretagogue sensitivity of Weibel-Palade bodies in endothelial cells.||Lui-Roberts WW,Ferraro F,Nightingale TD,Cutler DF||Molecular biology of the cell (19:5072)||2008|
|Not Applicable||Not Cited||Cytoskeletal remodeling in differentiated vascular smooth muscle is actin isoform dependent and stimulus dependent.||Kim HR,Gallant C,Leavis PC,Gunst SJ,Morgan KG||American journal of physiology. Cell physiology (295:C768)||2008|
|Not Applicable||Not Cited||Induction of muscle thermogenesis by high-fat diet in mice: association with obesity-resistance.||Kus V,Prazak T,Brauner P,Hensler M,Kuda O,Flachs P,Janovska P,Medrikova D,Rossmeisl M,Jilkova Z,Stefl B,Pastalkova E,Drahota Z,Houstek J,Kopecky J||American journal of physiology. Endocrinology and metabolism (295:E356)||2008|
|Not Applicable||Not Cited||Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations.||Pino MF,Kim KA,Shelton KD,Lindner J,Odili S,Li C,Collins HW,Shiota M,Matschinsky FM,Magnuson MA||The Journal of biological chemistry (282:13906)||2007|
|Not Applicable||Not Cited||Regulation of MAPK-activated protein kinase 5 activity and subcellular localization by the atypical MAPK ERK4/MAPK4.||Aberg E,Perander M,Johansen B,Julien C,Meloche S,Keyse SM,Seternes OM||The Journal of biological chemistry (281:35499)||2006|
|Not Applicable||Not Cited||Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IkappaBepsilon.||Stefansson B,Brautigan DL||The Journal of biological chemistry (281:22624)||2006|
|Not Applicable||Not Cited||Insulin antagonizes ischemia-induced Thr172 phosphorylation of AMP-activated protein kinase alpha-subunits in heart via hierarchical phosphorylation of Ser485/491.||Horman S,Vertommen D,Heath R,Neumann D,Mouton V,Woods A,Schlattner U,Wallimann T,Carling D,Hue L,Rider MH||The Journal of biological chemistry (281:5335)||2006|
|Not Applicable||Not Cited||Complexes between the LKB1 tumor suppressor, STRAD alpha/beta and MO25 alpha/beta are upstream kinases in the AMP-activated protein kinase cascade.||Hawley SA,Boudeau J,Reid JL,Mustard KJ,Udd L,Mäkelä TP,Alessi DR,Hardie DG||Journal of biology (2:null)||2004|
|Not Applicable||Not Cited||Increased phosphorylation of skeletal muscle glycogen synthase at NH2-terminal sites during physiological hyperinsulinemia in type 2 diabetes.||Højlund K,Staehr P,Hansen BF,Green KA,Hardie DG,Richter EA,Beck-Nielsen H,Wojtaszewski JF||Diabetes (52:1393)||2003|