Western blot analysis of Dopamine Receptor D1B was performed by loading 25 ug of C6 (lane 1) and SH-SY5Y (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Dopamine Receptor D1B monoclonal antibody (Product # MA1-46025) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~53 kDa.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant rat D1b dopamine receptor (within the last 118 of the C-terminus).|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Do not boil samples prior to Western Blot.
Suggested positive control: Sf9 cell lysates.
The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Decreased striatal dopamine receptor binding in primary focal dystonia: a D2 or D3 defect?
MA1-46025 was used in immunocytochemistry to investigate the relationship between the decrease of D3 receptor expression and primary focal dystonia
|Karimi M,Moerlein SM,Videen TO,Luedtke RR,Taylor M,Mach RH,Perlmutter JS||Movement disorders : official journal of the Movement Disorder Society (26:100)||2011|