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Immunofluorescent analysis of Dopamine Transporter in Lewis Lung carcinoma porcine kidney cells that were engineered to stably express the rat dopamine transporter, using a Dopamine Transporter monoclonal antibody (Product # MA5-18079) followed by detection using a FITC-conjugated secondary antibody.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic amino acid N-terminal peptide of rat dopamine transporter|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not cross-react with human samples.
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson"e;s disease and drug abuse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DA transporter; DAT; DAT1; dopamine transporter 1; sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; solute carrier family 6 member 3; solute carrier family 6, member 3
DAT; Dat1; Slc6a3