|Tested species reactivity||Chicken, Cat, Guinea pig, Human, Mouse, Pig, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG|
|Immunogen||Chicken drebrin E protein.|
|Storage buffer||PBS, pH 7.2, with 10mg/ml sucrose|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-20377 detects drebrin in human, mouse, rat, feline, porcine, chicken and guinea pig samples.
MA1-20377 has been successfully used in immunohistochemistry (frozen and paraffin) and Western blot procedures. A suggested positive control for this product is mouse brain tissue extract.
The MA1-20377 immunogen is chicken drebrin E protein.
Store at 4°C short term. For extended periods, store at -20°C, avoiding freeze/thaw cycles.
The cortical cytoskeleton, a highly cross-linked submembranal F-actin filament network, plays an important role in cell proliferation, differentiation, migration, cell morphology and oncogenic transformation. Drebrin (developmentally-regulated brain protein) is a major neuronal F-actin binding protein involved in the control of actin dynamics and neuronal morphogenesis. Three major isoforms of drebrin, resulting from alternative splicing of a single gene, have been identified in the brain: the embryonic isoforms designated E1 and E2 and the adult or A isoform (95-120 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Selective reduction of drebrin and actin in dendritic spines of hippocampal neurons by activation of 5-HT(2A) receptors.
MA1-20377 was used in immunocytochemistry to study the ability of 5-HT(2A) receptor activation to reduce drebrin and F-actin levels in hippocampal neuron dendritic spines
|Roppongi RT,Kojima N,Hanamura K,Yamazaki H,Shirao T||Neuroscience letters (547:76)||2013|