Immunofluorescent analysis of Dvl-2 showing staining in the cytoplasm of HCT 116 cells. HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Dvl-2 polyclonal antibody (Product # PA5-29244) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 43 and 315 of Human Dvl-2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29244 targets Dvl-2 in WB applications and shows reactivity with Human samples.
The PA5-29244 immunogen is recombinant fragment corresponding to a region within amino acids 43 and 315 of Human Dvl-2.
This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
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