|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide corresponding to the N-terminal of human Dynactin 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl, 50% glycerol|
|Contains||0.2% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous levels of DCTN1 at a molecular weight of 150 kDa and does not cross-react with related proteins.
Purity is >95% by SDS-PAGE.
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB which is also known as distal spinal and bulbar muscular atrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.