Immunofluorescence analysis of Dynamin I was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin I Rabbit Polyclonal Antibody (Product # PA1-660) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide corresponding to resdiues C K(633) E K A S E T E E N G S D S F(647) of rat Dyn1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-660 detects dynamin I (Dyn1) from rat tissues.
PA1-660 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn1 from rat whole brain extract.
PA1-660 immunizing peptide corresponds to amino acid residues 633-647 from rat Dyn1. This sequence is completely conserved between rat, mouse, and human Dyn1. PA1-660 immunizing peptide (Cat. # PEP-042) is available for use in neutralization and control experiments.
The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin I is expressed exclusively in neurons, dynamin II is ubiquitously expressed, and dynamin III is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dynamin 1 isoform roles in a mouse model of severe childhood epileptic encephalopathy.
PA1-660 was used in immunoprecipitation and western blot to characterize the role of dynamin 1 isoform using a severe epileptic encephalopathy mouse model
|Asinof S,Mahaffey C,Beyer B,Frankel WN,Boumil R||Neurobiology of disease (95:1)||2016|
Independent Neuronal Origin of Seizures and Behavioral Comorbidities in an Animal Model of a Severe Childhood Genetic Epileptic Encephalopathy.
PA1-660 was used in western blot to develop a conditional Dnm1Ftfl mouse model of epileptic encephalopathies.
|Asinof SK,Sukoff Rizzo SJ,Buckley AR,Beyer BJ,Letts VA,Frankel WN,Boumil RM||PLoS genetics (11:null)||2015|
Synaptic function is modulated by LRRK2 and glutamate release is increased in cortical neurons of G2019S LRRK2 knock-in mice.
PA1-660 was used in western blot to determine the effect of the G2019S mutation of LRRK2 has on the synaptic release of mammalian neurons
|Beccano-Kelly DA,Kuhlmann N,Tatarnikov I,Volta M,Munsie LN,Chou P,Cao LP,Han H,Tapia L,Farrer MJ,Milnerwood AJ||Frontiers in cellular neuroscience (8:null)||2014|
Parkinson's disease-linked mutations in VPS35 induce dopaminergic neurodegeneration.
PA1-660 was used in western blot to study the induction of dopaminergic neurodegeneration by VPS35 mutations associated with Parkinson's disease
|Tsika E,Glauser L,Moser R,Fiser A,Daniel G,Sheerin UM,Lees A,Troncoso JC,Lewis PA,Bandopadhyay R,Schneider BL,Moore DJ||Human molecular genetics (23:4621)||2014|
Dynamin 1 is required for memory formation.
PA1-660 was used in western blot to study the role of dynamin-1 in hippocampal-dependent associative memory
|Fà M,Staniszewski A,Saeed F,Francis YI,Arancio O||PloS one (9:null)||2014|
Protein partners of dynamin-1 in the retina.
PA1-660 was used in immunohistochemistry, immunoprecipitation, and western blot to identify protein binding partners of retinal dynamin-1
|Grossman GH,Ebke LA,Beight CD,Jang GF,Crabb JW,Hagstrom SA||Visual neuroscience (30:129)||2013|
Alzheimer brain-derived amyloid ß-protein impairs synaptic remodeling and memory consolidation.
PA1-660 was used in western blot to study the effect of water-soluble beta-amyloid extracts from Alzheimer's disease brains on synaptic remodeling and memory in recipient rats
|Borlikova GG,Trejo M,Mably AJ,Mc Donald JM,Sala Frigerio C,Regan CM,Murphy KJ,Masliah E,Walsh DM||Neurobiology of aging (34:1315)||2013|
A missense mutation in a highly conserved alternate exon of dynamin-1 causes epilepsy in fitful mice.
PA1-660 was used in western blot to study the role of a missense mutation in dynamin-1 in the pathogenesis of epilepsy in fitful mice
|Boumil RM,Letts VA,Roberts MC,Lenz C,Mahaffey CL,Zhang ZW,Moser T,Frankel WN||PLoS genetics (6:null)||2010|
Dynamin 1 depletion and memory deficits in rats treated with Abeta and cerebral ischemia.
PA1-660 was used in western blot to investigate the mechanism of memory impairment induced by Abeta and cerebral ischemia
|Watanabe T,Iwasaki K,Takasaki K,Yamagata N,Fujino M,Nogami A,Ii M,Katsurabayashi S,Mishima K,Fujiwara M||Journal of neuroscience research (88:1908)||2010|
Endocytosis in cultured neurons is altered by chronic alcohol exposure.
PA1-660 was used in western blot to investigate the influence of chronic alcohol consumption on neuronal endocytosis
|Marín MP,Esteban-Pretel G,Ponsoda X,Romero AM,Ballestín R,López C,Megías L,Timoneda J,Molowny A,Canales JJ,Renau-Piqueras J||Toxicological sciences : an official journal of the Society of Toxicology (115:202)||2010|
Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia.
PA1-660 was used in immunocytochemistry and western blot to demonstrate the role of a dynamin 1/cortactin ring complex for the maintenance of growth cone filopodia
|Yamada H,Abe T,Satoh A,Okazaki N,Tago S,Kobayashi K,Yoshida Y,Oda Y,Watanabe M,Tomizawa K,Matsui H,Takei K||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:4514)||2013|