|Tested species reactivity||Fish, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 500-600 of human DRP1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||2.5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2.5 µg/ml|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine based on 100% sequence homology.
Suggested positive control: antigen standard for DNM1L (transient overexpression lysate), human kidney protein.
A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death.
PA1-16987 was used in western blot to facilitate LIM kinase 2-mediated programmed necrotic neuronal death by mitochondrial translocation of high mobility group box 1
|Hyun HW,Ko AR,Kang TC||Frontiers in cellular neuroscience (10:null)||2016|
Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity.
PA1-16987 was used in western blot to study the role of endothelin-1 in LIMK2-mediated neuronal necrosis
|Ko AR,Hyun HW,Min SJ,Kim JE,Kang TC||Molecular brain (8:null)||2015|