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Immunofluorescent analysis of Dynein in U87-MG Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody (Product # MA1-070) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Many|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Purified bovine brain cytoplasmic dynein.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 ug/test|
|Immunocytochemistry (ICC)||2-4 µg/ml|
|Immunofluorescence (IF)||2-4 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-070 detects two isoforms of the ~74 kDa polypetide of cytoplasmic dynein.
MA1-070 has been successfully used in Western blot immunofluorescence, and immunoprecipitation procedures. In Western blot analysis of HeLa cell lysate this antibody detects a ~74 kDa protein representing cytoplasmic dynein.
The MA1-070 immunogen is purified bovine brain cytoplasmic dynein.
The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1.
MA1-070 was used in immunoprecipitation to investigate the regulation of cellular localization of mitochondria by dynein
|Varadi A,Johnson-Cadwell LI,Cirulli V,Yoon Y,Allan VJ,Rutter GA||Journal of cell science (117:4389)||2004|
Cytoplasmic dynein is associated with slow axonal transport.
MA1-070 was used in immunoprecipitation to investigate the relationship between dynein and slow axonal transport
|Dillman JF,Dabney LP,Pfister KK||Proceedings of the National Academy of Sciences of the United States of America (93:141)||1996|
Differential expression and phosphorylation of the 74-kDa intermediate chains of cytoplasmic dynein in cultured neurons and glia.
MA1-070 was used in western blot to investigate the brain cytoplasmic IC74 isoforms and their interaction with dynein
|Pfister KK,Salata MW,Dillman JF,Vaughan KT,Vallee RB,Torre E,Lye RJ||The Journal of biological chemistry (271:1687)||1996|
8 kDa dynein light chain b; cytosolic DH IC-1; DLC8b; dynein light chain 2, cytoplasmic; dynein light chain LC8-type 2; dynein light chain-2; dynein, light chain, LC8-type 2; radial spoke 22 homolog
1700064A15Rik; 6720463E02Rik; BOS_18355; C87222; DLC2; DLC8; DLC8b; DNCL1B; DYNLL2; RSPH22