Immunofluorescent analysis of cells using a Dynein Heavy Chain monoclonal antibody (Product # MA1-26770) after extraction with a detergent containing microtubule-stabilizing buffer. The image shows localization of cytoplasmic dynein to the mitotic spindle in dividing CEF cells. (C) Prometaphase. (F) Metaphase. (I) Anaphase.
|Tested species reactivity||Chicken, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytoplasmic, full length, native, chicken protein purified from chick brain.|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26770 detects Dynein heavy chain from chicken, human samples. MA1-26770 will not react with hamster CHO cell chromosomal dynein or dynein from Chlamydomonas flagella.
MA1-26770 has been successfully used in immunofluorescence, and Western blot procedures. By Western blot, MA1-26770 detects an ~440kD protein representing Dynein heavy chain.
The MA1-26770 immunogen is cytoplasmic, full length, native, chicken protein purified from chick brain.
This gene encodes the heavy chain subunit of axonemal dynein, a large multi-subunit molecular motor. Axonemal dynein attaches to microtubules and hydrolyzes ATP to mediate the movement of cilia and flagella. The gene expresses at least two transcript variants; additional variants have been described, but their full length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.