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Immunofluorescent analysis of cells using a Dynein Heavy Chain monoclonal antibody (Product # MA1-26770) after extraction with a detergent containing microtubule-stabilizing buffer. The image shows localization of cytoplasmic dynein to the mitotic spindle in dividing CEF cells. (C) Prometaphase. (F) Metaphase. (I) Anaphase.
|Tested species reactivity||Chicken, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytoplasmic, full length, native, chicken protein purified from chick brain.|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26770 detects Dynein heavy chain from chicken, human samples. MA1-26770 will not react with hamster CHO cell chromosomal dynein or dynein from Chlamydomonas flagella.
MA1-26770 has been successfully used in immunofluorescence, and Western blot procedures. By Western blot, MA1-26770 detects an ~440kD protein representing Dynein heavy chain.
The MA1-26770 immunogen is cytoplasmic, full length, native, chicken protein purified from chick brain.
This gene encodes the heavy chain subunit of axonemal dynein, a large multi-subunit molecular motor. Axonemal dynein attaches to microtubules and hydrolyzes ATP to mediate the movement of cilia and flagella. The gene expresses at least two transcript variants; additional variants have been described, but their full length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
axonemal beta dynein heavy chain 9; ciliary dynein heavy chain 9; DNAH17L; DNAH9 variant protein; Dnahc9; DNEL1; DYH9; dynein, axonemal, heavy polypeptide 9; HL-20; HL20; KIAA0357
DNAH17L; DNAH9; Dnahc9; DNAL1; DNEL1; DYH9; HL-20; HL20; KIAA0357