|Tested species reactivity||Chicken, Deer, Hamster, Human, Rat|
|Host / Isotype||Mouse / IgM|
|Immunogen||Cytoplasmic, full length, native, chicken dynein intermediate chain protein purified from chick brain.|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22146 detects Dynein intermediate chains in human, indian muntjac (deer), rat, chicken, and hamster samples. This antibody is know to detect cytoplasmic Dynein intermediate chains, and reactivity with the axonemal form is not known.
MA1-22146 has been successfully used in ELISA, immunocytochemistry, immunofluorescence, immunoprecipitation and Western blot procedures. By Western blot, MA1-22146 detects a ~ 70 kDa triplet representing the Dynein intermediate chains.
The MA1-22146 immunogen is cytoplasmic, full length, native, chicken dynein intermediate chain protein purified from chick brain.
Eukaryotic cells rely on actin and microtubule-based protein ""motors"" to generate intracellular movements. These protein ""motors"" contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.