Immunohistochemical analysis of Dysadherin in paraffin-embedded human tonsil tissue using a Dysadherin polyclonal antibody (Product # PA1-31506) at a dilution of 0.3µg/ml. Microwaved antigen retrieval was performed using a citrate buffer, pH 6.0, followed by HRP-staining.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide GKCRQLSRLCRNHCR (Human).|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||0.5-1.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31506 detects Dysadherin from human samples.
PA1-31506 has been successfully used in Western blot applications.
The PA1-31506 immunogen is: Synthetic peptide: GKCRQLSRLCRNHCR (Human).
This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis, and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants. [RefSeq curation by Kathleen J. Sweadner, Ph.D., firstname.lastname@example.org.]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.