IHC analysis of horse muscle tissues using PA1-37587. Horse muscle sections (8µm) were blocked with 5% horse serum in 1X PBS and stained with PA1-37587 (1:50) for 1 hour followed by a fluorophore-conjugated anti-rabbit IgG secondary antibody (red). Tissues were also stained for Myosin Heavy chain type IIA (green). Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from c-terminus of human dystrophin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
This antibody is predicted to react with bovine, chicken, canine and porcine based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skeletal muscle tissue.
Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne's muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Realimentation of nutrient restricted pregnant beef cows supports compensatory fetal muscle growth.
PA1-37587 was used in immunohistochemistry - frozen section to study the effect of nutrient restriction at specific gestational timeframes on fetal skeletal muscle growth
|Gonzalez JM,Camacho LE,Ebarb SM,Swanson KC,Vonnahme KA,Stelzleni AM,Johnson SE||Journal of animal science (91:4797)||2013|