|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 2 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Bovine , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from c-terminus of human dystrophin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with bovine, chicken, canine and porcine based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skeletal muscle tissue.
Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne's muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; Dystrophin; dystrophin Dp40; dystrophin Dp71a; dystrophin Dp71ab; dystrophin Dp71b; GS1-19O24.1; X-linked muscular dystrophy
Gene Aliases: BMD; CMD3B; DMD; Dp427; Dp71; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; DXSmh7; DXSmh9; dys; mdx; MRX85; pke
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