|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to C-terminal of human Dystrophin|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunohistochemistry (IHC)||See 2 publications below|
PA5-16734 targets Dystrophin in IHC (P) applications and shows reactivity with Human and Rat samples.
The PA5-16734 immunogen is a synthetic peptide corresponding to C-terminal of human Dystrophin.
Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immuohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.
Recovery of diaphragm function following mechanical ventilation in a rodent model.
PA5-16734 was used in immunohistochemistry to use a rodent model to study the time-course of recovery of diaphragm muscle fiber atrophy and contractile dysfunction following return to voluntary breathing after mechanical ventilation
|Bruells CS,Bergs I,Rossaint R,Du J,Bleilevens C,Goetzenich A,Weis J,Wiggs MP,Powers SK,Hein M||PloS one (9:null)||2014|
Variable phenotype of del45-55 Becker patients correlated with nNOSμ mislocalization and RYR1 hypernitrosylation.
PA5-16734 was used in immunohistochemistry to study the correlation of del45-55 Becker muscular dystrophy phenotype with nNOSmu mislocalization and RYR1 hypernitrosylation
|Gentil C,Leturcq F,Ben Yaou R,Kaplan JC,Laforet P,Pénisson-Besnier I,Espil-Taris C,Voit T,Garcia L,Piétri-Rouxel F||Human molecular genetics (21:3449)||2012|
apodystrophin-3; apodystrophin-I; dystrophin; dystrophin, muscular dystrophy; OTTHUMP00000215592
BMD; CMD3B; DMD; DNADMD1; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85