Invitrogen

Dystrophin Polyclonal Antibody

2 Published Figures

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Dystrophin Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human skeletal muscle stained with Dystrophin antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of muscle fibers.

Dystrophin Antibody — 2 FIGURE A, The top panel shows representative curves of maximal isometric torque (200 ms in duration) before injury (pre) and after injury (post) for quadriceps (QUAD) and TA muscle of WT and Dmd-KO rats. The bottom panels show representative trace recordings of torque from lengthening (eccentric) contractions. Muscles were stimulated for 200 ms to induce a peak isometric contraction before lengthening by movement of knee (QUAD) or ankle (TA) in the opposite direction of muscle's action at that joint. B, Scatter plots describing muscle mass, maximal isometric torque, and susceptibility to injury for TA (top) and Quad (bottom) in WT and Dmd-KO rats. * = Significance at P < .05 [Color figure can be viewed at wileyonlinelibrary.com ]

Dystrophin Antibody — Figure 1 The timeline of clinical information, results of the ischemic forearm exercise test, IHC using dystrophin C-terminal antibody, and genetic analysis. (a) Clinical complaints and the results of the blood test corresponding to symptoms are depicted depending on the age. (b) Exercise-associated lactate and ammonia production is identified. (c) Mildly increased fiber size variation and increased number of fibers with internal nuclei are seen in hematoxylin and eosin stain. Absence of immune activity against dystrophin C-terminal antibody. Scale bar, 100 mum. (d) The mutation c.119T > A (p.Leu40His, NM_004006.2) in the DMD gene is confirmed by Sanger sequence in both patient and mother. AST, aspartate transaminase, U/L; ALT, alanine transaminase, U/L; CK, creatine kinase; U/L; myoglobin, ng/mL

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

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Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

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View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Artificial Control, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to C-terminal of human Dystrophin

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C

RRID

AB_10978300

Product Specific Information

PA5-16734 targets Dystrophin in IHC (P) applications and shows reactivity with Human and Rat samples.

The PA5-16734 immunogen is a synthetic peptide corresponding to C-terminal of human Dystrophin.

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: apodystrophin-3; apodystrophin-I; Dystrophin; dystrophin, muscular dystrophy; GS1-19O24.1; OTTHUMP00000215592

Gene Aliases: BMD; CMD3B; DMD; DNADMD1; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85

UniProt ID: (Human) P11532, (Rat) P11530

Entrez Gene ID: (Human) 1756, (Rat) 24907

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein

regulation of heart rate cytoskeleton organization muscle organ development regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion regulation of skeletal muscle contraction muscle attachment muscle filament sliding negative regulation of peptidyl-serine phosphorylation cellular protein localization response to muscle stretch peptide biosynthetic process cellular protein complex assembly muscle cell cellular homeostasis muscle fiber development cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity cardiac muscle cell action potential regulation of voltage-gated calcium channel activity negative regulation of peptidyl-cysteine S-nitrosylation positive regulation of sodium ion transmembrane transporter activity positive regulation of cell-matrix adhesion regulation of transcription, DNA-templated skeletal muscle tissue development aging establishment of blood-nerve barrier regulation of gene expression positive regulation of neuron projection development myotube cell development olfactory nerve structural organization cell differentiation regulation of membrane potential muscle cell differentiation neurotransmitter receptor metabolic process positive regulation of neuron differentiation neuron projection morphogenesis nucleus localization establishment of glial blood-brain barrier negative regulation of ERK1 and ERK2 cascade regulation of cellular response to growth factor stimulus

Process(es)

dystroglycan binding actin binding structural constituent of cytoskeleton protein binding zinc ion binding structural constituent of muscle myosin binding vinculin binding nitric-oxide synthase binding integrin binding PDZ domain binding protein complex binding