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      Dystrophin Polyclonal Antibody

      See 7 other Dystrophin antibodies ›
      Dystrophin Polyclonal Antibody for IHC (P)

      Catalog#: PA5-16734 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA5-16734 500 µL
      309.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human, Rat
      Published species reactivity Rat, Mouse, Human, Not Applicable
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen A synthetic peptide corresponding to C-terminal of human Dystrophin
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.2 mg/ml
      Purification Affinity chromatography
      Storage buffer PBS, pH 7.6, with 0.2% BSA
      Contains 15mM sodium azide
      Storage Conditions 4° C
      Tested Applications Dilution *
      Immunohistochemistry (Paraffin) (IHC (P)) 1:200-1:400

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Immunohistochemistry (IHC) See 4 publications below
      Miscellaneous PubMed (MISC) See 1 publications below
      Immunohistochemistry (Frozen) (IHC (F)) See 1 publications below

      Product Specific Information

      PA5-16734 targets Dystrophin in IHC (P) applications and shows reactivity with Human and Rat samples.

      The PA5-16734 immunogen is a synthetic peptide corresponding to C-terminal of human Dystrophin.

      Background/Target Information

      Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immuohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Immunohistochemistry

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable Not Cited Targeted Expression of Catalase to Mitochondria Protects Against Ischemic Myopathy in High-Fat Diet-Fed Mice.
      PA5-16734 was used in immunohistochemistry to study protection against ischemic myopathy in high fat fed mice by targeted expression of catalase to mitochondria       		Ryan TE,Schmidt CA,Green TD,Spangenburg EE,Neufer PD,McClung JM Diabetes (65:2553) 2016
      Rat Not Cited Recovery of diaphragm function following mechanical ventilation in a rodent model.
      PA5-16734 was used in immunohistochemistry to use a rodent model to study the time-course of recovery of diaphragm muscle fiber atrophy and contractile dysfunction following return to voluntary breathing after mechanical ventilation       		Bruells CS,Bergs I,Rossaint R,Du J,Bleilevens C,Goetzenich A,Weis J,Wiggs MP,Powers SK,Hein M PloS one (9:null) 2014
      Not Applicable Not Cited Obscurin is required for ankyrinB-dependent dystrophin localization and sarcolemma integrity.
      PA5-16734 was used in immunohistochemistry to characterize mice deficient for obscurin       		Randazzo D,Giacomello E,Lorenzini S,Rossi D,Pierantozzi E,Blaauw B,Reggiani C,Lange S,Peter AK,Chen J,Sorrentino V The Journal of cell biology (200:523) 2013
      Human 1:500 Variable phenotype of del45-55 Becker patients correlated with nNOS¿ mislocalization and RYR1 hypernitrosylation.
      PA5-16734 was used in immunohistochemistry to study the correlation of del45-55 Becker muscular dystrophy phenotype with nNOSmu mislocalization and RYR1 hypernitrosylation       		Gentil C,Leturcq F,Ben Yaou R,Kaplan JC,Laforet P,Pénisson-Besnier I,Espil-Taris C,Voit T,Garcia L,Piétri-Rouxel F Human molecular genetics (21:3449) 2012

      Miscellaneous PubMed

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse Not Cited The first exon duplication mouse model of Duchenne muscular dystrophy: A tool for therapeutic development.
      PA5-16734 was used in western blot to generate and characterize a mouse model that carries a Dmd exon 2 duplication       		Vulin A,Wein N,Simmons TR,Rutherford AM,Findlay AR,Yurkoski JA,Kaminoh Y,Flanigan KM Neuromuscular disorders : NMD (25:827) 2015

      Immunohistochemistry (Frozen)

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable Not Cited Recovery of altered neuromuscular junction morphology and muscle function in mdx mice after injury.
      PA5-16734 was used in immunohistochemistry - frozen section to investigate mdx mice after injury and recovery of altered neuromuscular junction morphology and muscle function       		Pratt SJ,Shah SB,Ward CW,Kerr JP,Stains JP,Lovering RM Cellular and molecular life sciences : CMLS (72:153) 2015

      Bioinformatics

      Protein Aliases:

      apodystrophin-3; apodystrophin-I; Dystrophin; dystrophin, muscular dystrophy; OTTHUMP00000215592

      Gene Aliases:

      BMD; CMD3B; DMD; DNADMD1; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85

      UniProt ID:

      (Human) P11532, (Rat) P11530

      Entrez Gene ID:

      (Human) 1756, (Rat) 24907

      Molecular Function:

      actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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