Invitrogen

Dystrophin Polyclonal Antibody

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Dystrophin Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human skeletal muscle stained with Dystrophin antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of muscle fibers.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

Published Applications

Immunohistochemistry (IHC)

See 4 publications below

Miscellaneous PubMed (MISC)

See 1 publication below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to C-terminal of human Dystrophin

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C

RRID

AB_10978300

Product Specific Information

PA5-16734 targets Dystrophin in IHC (P) applications and shows reactivity with Human and Rat samples.

The PA5-16734 immunogen is a synthetic peptide corresponding to C-terminal of human Dystrophin.

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immuohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: apodystrophin-3; apodystrophin-I; Dystrophin; dystrophin, muscular dystrophy; OTTHUMP00000215592

Gene Aliases: BMD; CMD3B; DMD; DNADMD1; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85

UniProt ID: (Human) P11532, (Rat) P11530

Entrez Gene ID: (Human) 1756, (Rat) 24907

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein

regulation of heart rate cytoskeleton organization muscle organ development regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion regulation of skeletal muscle contraction muscle attachment muscle filament sliding negative regulation of peptidyl-serine phosphorylation cellular protein localization response to muscle stretch peptide biosynthetic process cellular protein complex assembly muscle cell cellular homeostasis muscle fiber development cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity cardiac muscle cell action potential regulation of voltage-gated calcium channel activity negative regulation of peptidyl-cysteine S-nitrosylation positive regulation of sodium ion transmembrane transporter activity positive regulation of cell-matrix adhesion regulation of transcription, DNA-templated skeletal muscle tissue development aging establishment of blood-nerve barrier regulation of gene expression positive regulation of neuron projection development myotube cell development olfactory nerve structural organization cell differentiation regulation of membrane potential muscle cell differentiation neurotransmitter receptor metabolic process positive regulation of neuron differentiation neuron projection morphogenesis nucleus localization establishment of glial blood-brain barrier negative regulation of ERK1 and ERK2 cascade regulation of cellular response to growth factor stimulus

Process(es)

dystroglycan binding actin binding structural constituent of cytoskeleton protein binding zinc ion binding structural constituent of muscle myosin binding vinculin binding nitric-oxide synthase binding integrin binding PDZ domain binding protein complex binding