|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||LPS-activated mlEND1 cells expressing E-selectin at their cell surface.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:500|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06507 detects E-selectin in mouse samples.
MA1-06507 has sucessfully been used in flow cytometry, immunocytochemistry, immunofluorescence, Western blotting, immunoprecipitation, immunohistochemistry and ELISA.
The MA1-06507 immunogen is LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.