|Tested species reactivity||Human, Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human E-Selectin (ELAM-1).|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||10 ug/ml|
|Flow Cytometry (Flow)||10 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1 - 10 ug/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-80430 inhibits neutrophil adhesion to E-selectin and to P-selectin.
Non-reducing conditions are recommended for Western blot applications. A suggested positive control is human tonsil. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD62E is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. CD62P cell surface antigen is a 140kDa glycoprotein also known as P-selectin. CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.