Invitrogen

E-cadherin Polyclonal Antibody

E-cadherin Antibody in Immunocytochemistry (ICC/IF)

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E-cadherin Antibody (PA5-32178) in ICC/IF

Immunofluorescence analysis of E-cadherin was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with E-cadherin Polyclonal Antibody (Product # PA5-32178) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ce... View More

E-cadherin Antibody in Western Blot (WB)

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Product Details

PA5-32178

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 4 publications 4 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 338 and 649 of Human E-cadherin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.09 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2549651

Product Specific Information

Recommended positive controls: 293T, PC-12, MCF-7.

Predicted reactivity: Rat (80%), Pig (82%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CircPRDM5 inhibits the proliferation, migration, invasion, and glucose metabolism of gastric cancer cells by reducing GCNT4 expression in a miR-485-3p-dependent manner.

The Kaohsiung journal of medical sciences

Lan ZZ,Sun FH,Chen C,Niu L,Shi JD,Zhang WY

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Fri Mar 01 00:00:00 UTC 2024

CircMETTL15 induces TMTC3 production by absorbing miR-944 to promote hepatocellular carcinoma cell malignancy.

Journal of biochemical and molecular toxicology

Zhao Y,Chen J,Xu S,Chen Y

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon Jan 01 00:00:00 UTC 2024

Development of rat duodenal monolayer model with effective barrier function from rat organoids for ADME assay.

Scientific reports

Tanaka K,Kawai S,Fujii E,Yano M,Miyayama T,Nakano K,Terao K,Suzuki M

PA5-32178 was used in Immunohistochemistry to expect our novel monolayer model to be a useful tool for elucidating drug absorption processes in detail, enabling the development of highly absorbable drugs.

Wed Jul 26 00:00:00 UTC 2023

An immunoresponsive three-dimensional urine-tolerant human urothelial model to study urinary tract infection.

Frontiers in cellular and infection microbiology

Jafari NV,Rohn JL

PA5-32178 was used in Immunocytochemistry to develop a human urothelial three-dimensional (3D) model which was urine tolerant and demonstrated several urothelial biomarkers, but it only achieved human thickness in heterogenous, multi-layered zones and did not demonstrate the comprehensive differentiation status needed to achieve barrier function.

Fri Apr 14 00:00:00 UTC 2023

Re-expression of circ_0043610 contributes to trophoblast dysfunction through the miR-558/RYBP pathway in preeclampsia.

Endocrine journal

Shang J,Lin L,Huang X,Zhou L,Huang Q

PA5-32178 was used in Western Blotting to investigate the function and the potential mechanism of circ_0043610 in preeclampsia.

Wed Dec 28 00:00:00 UTC 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Wed Feb 02 00:00:00 UTC 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Wed Feb 02 00:00:00 UTC 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Wed Jun 01 00:00:00 UTC 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Wed Jun 01 00:00:00 UTC 2022

Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer Cells.

Molecules (Basel, Switzerland)

Wang H,Luo Y,Chu Z,Ni T,Ou S,Dai X,Zhang X,Liu Y

PA5-32178 was used in Western Blotting to show that Triterpenoids from P. cocos have significant biological activity against gastric cancer, and the mechanism may be involved in the process of epithelial-mesenchymal transformation.

Mon Jun 06 00:00:00 UTC 2022

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon Jun 21 00:00:00 UTC 2021

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon Jun 21 00:00:00 UTC 2021

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon Jun 21 00:00:00 UTC 2021

RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers.

Frontiers in oncology

Abraham HG,Ulintz PJ,Goo L,Yates JA,Little AC,Bao L,Wu Z,Merajver SD

PA5-32178 was used in Western Blot, Immunohistochemistry to study how RhoA- and RhoC-GTPase influence the cell-cell junctions in aggressive breast cancers.

Tue Nov 07 00:00:00 UTC 2023

RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers.

Frontiers in oncology

Abraham HG,Ulintz PJ,Goo L,Yates JA,Little AC,Bao L,Wu Z,Merajver SD

PA5-32178 was used in Western Blot, Immunohistochemistry to study how RhoA- and RhoC-GTPase influence the cell-cell junctions in aggressive breast cancers.

Tue Nov 07 00:00:00 UTC 2023

E2F5 Promotes the Malignancy of Ovarian Cancer Via the Regulation of Hippo and Wnt Pathways.

Genetic testing and molecular biomarkers

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-32178 was used in Western Blot to conclude that E2F5 promotes ovarian carcinogenesis via the regulation of Hippo and Wnt pathways.

Mon Mar 01 00:00:00 UTC 2021

Monitoring Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction.

Methods and protocols

Sim JJ,Jeong KY

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Immunocytochemistry

Mon Apr 27 00:00:00 UTC 2020

Monitoring Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction.

Methods and protocols

Sim JJ,Jeong KY

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Immunocytochemistry

Mon Apr 27 00:00:00 UTC 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon May 04 00:00:00 UTC 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon May 04 00:00:00 UTC 2020

Biophysical Control of Bile Duct Epithelial Morphogenesis in Natural and Synthetic Scaffolds.

Frontiers in bioengineering and biotechnology

Funfak A,Bouzhir L,Gontran E,Minier N,Dupuis-Williams P,Gobaa S

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Immunocytochemistry

Wed Sep 30 00:00:00 UTC 2020

Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.

International journal of molecular sciences

Kao HH,Kuo CY,Chen KS,Chen JP

PA5-32178 was used in Immunohistochemistry-immunofluorescence to explore the possibility to culture mesothelial cells in a three-dimensional scaffold for tissue engineering applications.

Thu Sep 12 00:00:00 UTC 2019

Secreted Giardia intestinalis cysteine proteases disrupt intestinal epithelial cell junctional complexes and degrade chemokines.

Virulence

Liu J,Ma'ayeh S,Peirasmaki D,Lundström-Stadelmann B,Hellman L,Svärd SG

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon Dec 31 00:00:00 UTC 2018

Tumor bioengineering using a transglutaminase crosslinked hydrogel.

PloS one

Fang JY,Tan SJ,Yang Z,Tayag C,Han B

PA5-32178 was used in immunohistochemistry to develop a 3D culturing system to mimic the tumor microenvironment using transglutaminase-cross-linked gelatin gel (Col-Tgel)

Mon Dec 07 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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Gene Aliases: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO

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UniProt ID: (Human) P12830, (Rat) Q9R0T4

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Entrez Gene ID: (Human) 999, (Rat) 83502

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Function(s)

glycoprotein binding calcium ion binding protein binding beta-catenin binding ankyrin binding GTPase activating protein binding gamma-catenin binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion protein phosphatase binding protein domain specific binding cadherin

Process(es)

homophilic cell adhesion via plasma membrane adhesion molecules synapse assembly response to toxic substance single organismal cell-cell adhesion pituitary gland development negative regulation of cell-cell adhesion extracellular matrix disassembly extracellular matrix organization neuron projection development adherens junction organization response to drug positive regulation of transcription factor import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane establishment of protein localization to plasma membrane in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis sensory perception of sound response to organic substance calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules protein metabolic process regulation of protein localization regulation of water loss via skin regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus negative regulation of canonical Wnt signaling pathway cochlea development regulation of protein localization to cell surface regulation of neuron migration

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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E-cadherin Polyclonal Antibody

E-cadherin Antibody (PA5-32178) in ICC/IF

Product Details

PA5-32178

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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