Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Donryu rat after immunization with mouse teratocarcinoma and mouse liver E-cadherin fragment.
Specificity: This antibody specifically reacts with mouse E-cadherin. This antibody does not inhibit E-cadherin-dependent cell-cell contact.
Cross reactivity: This antibody reacts with human3 and dog E-cadherin. This antibody does not react with bovine or chicken E-cadherin.
Storage: 4°C This product does not contain preservative. The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Ca2+ should be contained in the buffers to stabilize cadherin antigen.
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ARC-1; cadherin 1; Cadherin-1; cadherin-E; calcium-dependent adhesion protein; CD324; CDHE; cell-CAM 120/80; E-Cadherin; E-cadherin epithelial; ECAD; epithelial; Epithelial cadherin; LCAM; UVO; Uvomorulin
Gene Aliases: AA960649; Cdh1; E-cad; Ecad; L-CAM; Um; UVO
UniProt ID: (Mouse) P09803
Entrez Gene ID: (Mouse) 12550
Molecular Function: cadherin