Immunofluorescence analysis of E-cadherin/CDH1 was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with E-cadherin/CDH1 [DH01 (DCS-266)] Mouse Monoclonal Antibody (MA511496) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human cdh1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 8 publications below|
MA5-11496 targets APC/C activator protein CDH1 in IP, ICC/IF and WB applications and shows reactivity with Human samples.
The MA5-11496 immunogen is recombinant human cdh1 protein.
Research Areas: Cell Cycle; Cdh1 protein is a limiting, substrate-specific activator of anaphase-promoting complex (APC)-dependent proteolysis and it is required for the degradation of the APC substrates, such as Clb2 and Ase1. Cdh1 plays important role in mitotic regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20.
MA5-11496 was used in western blot to explore how Nek2 isoform A affects anaphase-promoting complex/cyclosome containing Cdc20.
|Boekhout M,Wolthuis R||Journal of cell science (128:1639)||2015|
MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C.
MA5-11496 was used in western blot to study the effect of MASTL on cyclin B1 degradation and its mechanism
|Voets E,Wolthuis R||Biology open (4:484)||2015|
The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells.
MA5-11496 was used in western blot to study the role of Cdh1 in regulating G2/M transition during placental differentiation
|Naoe H,Chiyoda T,Ishizawa J,Masuda K,Saya H,Kuninaka S||Biochemical and biophysical research communications (430:757)||2013|
Skp1-Cul1-F-box ubiquitin ligase (SCF(ßTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry.
MA5-11496 was used in western blot to study the role of Skp1-Cul1-F-box ubiquitin ligase in regulating the levels of USP37 and cell cycle progression
|Burrows AC,Prokop J,Summers MK||The Journal of biological chemistry (287:39021)||2012|
Retinoblastoma protein and anaphase-promoting complex physically interact and functionally cooperate during cell-cycle exit.
MA5-11496 was used in western blot to examine the interaction between retinoblastoma protein and anaphase-promoting complex
|Binné UK,Classon MK,Dick FA,Wei W,Rape M,Kaelin WG,Näär AM,Dyson NJ||Nature cell biology (9:225)||2007|
Inactivation of human MAD2B in nasopharyngeal carcinoma cells leads to chemosensitization to DNA-damaging agents.
MA5-11496 was used in western blot to study the role of MAD2B in cellular sensitivity to DNA-damaging anticancer drugs in nasopharyngeal carcinoma cells
|Cheung HW,Chun AC,Wang Q,Deng W,Hu L,Guan XY,Nicholls JM,Ling MT,Chuan Wong Y,Tsao SW,Jin DY,Wang X||Cancer research (66:4357)||2006|
Human cytomegalovirus inactivates the G0/G1-APC/C ubiquitin ligase by Cdh1 dissociation.
MA5-11496 was used in western blot to study the mechanism by which human cytomegalovirus inactivates the G0/G1-APC/C ubiquitin ligase
|Wiebusch L,Bach M,Uecker R,Hagemeier C||Cell cycle (Georgetown, Tex.) (4:1435)||2005|
DNA damage-induced mitotic catastrophe is mediated by the Chk1-dependent mitotic exit DNA damage checkpoint.
MA5-11496 was used in western blot to study the role of Chk1 in mediating DNA damage-induced mitotic catastrophe
|Huang X,Tran T,Zhang L,Hatcher R,Zhang P||Proceedings of the National Academy of Sciences of the United States of America (102:1065)||2005|