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Immunofluorescence analysis of E-cadherin / CDH1 was done on 90% confluent log phase Caco2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with E-cadherin / CDH1 Antibody (EP700Y) Rabbit Polyclonal Antibody (MA514458) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit|
|Immunogen||A synthetic peptide derived from the fifth cadherin domain of human cadherin|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA5-14458 targets E-Cadherin in IHC (P) applications and shows reactivity with Human samples.
The MA5-14458 immunogen is a synthetic peptide derived from the fifth cadherin domain of human cadherin.
E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominantly in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Numerous studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis. E-cadherin is used in distinguishing LCIS from DCIS and that of ILC from IDC in indeterminate cases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Knock-down of plasminogen-activator inhibitor-1 enhances expression of E-cadherin and promotes epithelial differentiation of human pancreatic adenocarcinoma cells.
MA5-14458 was used in immunocytochemistry to study the increased E-cadherin expression and epithelial differentiation of human pancreatic carcinoma cells in response to PAI-1 deletion
|Lupu-Meiri M,Geras-Raaka E,Lupu R,Shapira H,Sandbank J,Segal L,Gershengorn MC,Oron Y||Journal of cellular physiology (227:3621)||2012|
Arc-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin-1; cadherin-E; calcium-dependent adhesion protein; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDHE; cell-CAM 120/80; E-Cadherin; E-cadherin 1; E-cadherin epithelial; ECAD; epithelial; epithelial cadherin; LCAM; UVO; uvomorulin
Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO