|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant protein encoding human cadherin-E aa 600-707|
|Storage buffer||TBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast, tonsil or skin tissue.
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Frequent aerogenous spread with decreased E-cadherin expression of ROS1-rearranged lung cancer predicts poor disease-free survival.
MA1-37202 was used in immunohistochemistry to characterize ROS1-rearranged tumors
|Jin Y,Sun PL,Park SY,Kim H,Park E,Kim G,Cho S,Kim K,Lee CT,Chung JH||Lung cancer (Amsterdam, Netherlands) (89:343)||2015|
Comparison of the clinicopathological features of invasive ductal, invasive lobular, and mixed (invasive ductal + invasive lobular) carcinoma of the breast.
MA1-37202 was used in immunohistochemistry to perform a retrospective study of the clinicopathology of various forms of invasive breast carcinoma
|Zengel B,Yararbas U,Duran A,Uslu A,El¿yatk¿n N,Dem¿rk¿ran MA,Cengiz F,¿im¿ek C,Postac¿ H,Vardar E,Durusoy R||Breast cancer (Tokyo, Japan) (22:374)||2015|
A comprehensive comparative analysis of the histomorphological features of ALK-rearranged lung adenocarcinoma based on driver oncogene mutations: frequent expression of epithelial-mesenchymal transition markers than other genotype.
MA1-37202 was used in immunohistochemistry to perform a detailed comparative analysis of ALK-rearranged and ALK-negative lung adenocarcinomas
|Kim H,Jang SJ,Chung DH,Yoo SB,Sun P,Jin Y,Nam KH,Paik JH,Chung JH||PloS one (8:null)||2013|
Epithelial-mesenchymal transition in breast cancer correlates with high histological grade and triple-negative phenotype.
MA1-37202 was used in immunohistochemistry to study the correlation of epithelial-mesenchymal transition with triple negative phenotype and high histological grade in breast cancer
|Jeong H,Ryu YJ,An J,Lee Y,Kim A||Histopathology (60:E87)||2012|
Down-regulation of microRNAs of the miR-200 family and miR-205, and an altered expression of classic and desmosomal cadherins in spindle cell carcinoma of the head and neck--hallmark of epithelial-mesenchymal transition.
MA1-37202 was used in immunohistochemistry to study the downregulation of specific microRNAs and altered expression of cadherins in spindle cell carcinoma of the head and neck
|Zidar N,Bo¿tjan¿i¿ E,Gale N,Kojc N,Poljak M,Glava¿ D,Cardesa A||Human pathology (42:482)||2011|
Apoptosis in the developing human heart resembles apoptosis in epithelial tissues.
MA1-37202 was used in immunohistochemistry to investigate the process of apoptosis in human heart during development
|Jer¿e M,Zidar N||Cell and tissue research (343:537)||2011|
GLI2-mediated melanoma invasion and metastasis.
MA1-37202 was used in immunohistochemistry to study the role of the Hedgehog pathway mediator GLI2 in regulating melanoma invasion and metastasis
|Alexaki VI,Javelaud D,Van Kempen LC,Mohammad KS,Dennler S,Luciani F,Hoek KS,Juàrez P,Goydos JS,Fournier PJ,Sibon C,Bertolotto C,Verrecchia F,Saule S,Delmas V,Ballotti R,Larue L,Saiag P,Guise TA,Mauviel A||Journal of the National Cancer Institute (102:1148)||2010|
An immunohistochemical study of colon adenomas and carcinomas: E-cadherin, Syndecan-1, Ets-1.
MA1-37202 was used in immunohistochemistry to investigate the immunohistochemical differences between colon adenomas and carcinomas
|Pap Z,Pávai Z,Dénes L,Kovalszky I,Jung J||Pathology oncology research : POR (15:579)||2009|
Cadherin-catenin complex and transcription factor Snail-1 in spindle cell carcinoma of the head and neck.
MA1-37202 was used in immunohistochemistry to examine the role of transcription factor Snail-1 in spindle cell carcinoma of the head and neck
|Zidar N,Gale N,Kojc N,Volavsek M,Cardesa A,Alos L,Höfler H,Blechschmidt K,Becker KF||Virchows Archiv : an international journal of pathology (453:267)||2008|
Immunohistochemical expression of CD44 standard and E-cadherin in atypical leiomyoma and leiomyosarcoma of the uterus.
MA1-37202 was used in immunohistochemistry - paraffin section to assess the diagnostic role of CD44 and E-cadherin in atypical leiomyoma and leiomyosarcoma.
|Kaygusuz EI||Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology (35:279)||2015|
The effects of sonic hedgehog signaling pathway components on non-small-cell lung cancer progression and clinical outcome.
MA1-37202 was used in immunohistochemistry - paraffin section to study non-small-cell lung cancer progression and clinical outcome by the effects of sonic hedgehog signaling pathway components
|Hwang J,Kang MH,Yoo YA,Quan YH,Kim HK,Oh SC,Choi YH||World journal of surgical oncology (12:null)||2014|