Immunofluorescence analysis of E-cadherin / CDH1 was done on 70% confluent log phase Caco2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled E-cadherin / CDH1 (SPM471) Mouse Monoclonal Antibody (MA514408) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant protein encoding human Cadherin-E 600-707aa|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14408 targets E-Cadherin in IHC (P) applications and shows reactivity with Human samples.
The MA5-14408 immunogen is recombinant protein encoding human Cadherin-E 600-707aa.
E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Numerous studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Frequent aerogenous spread with decreased E-cadherin expression of ROS1-rearranged lung cancer predicts poor disease-free survival.
MA5-14408 was used in immunohistochemistry to characterize ROS1-rearranged tumors
|Jin Y,Sun PL,Park SY,Kim H,Park E,Kim G,Cho S,Kim K,Lee CT,Chung JH||Lung cancer (Amsterdam, Netherlands) (89:343)||2015|
Comparison of the clinicopathological features of invasive ductal, invasive lobular, and mixed (invasive ductal + invasive lobular) carcinoma of the breast.
MA5-14408 was used in immunohistochemistry to perform a retrospective study of the clinicopathology of various forms of invasive breast carcinoma
|Zengel B,Yararbas U,Duran A,Uslu A,El¿yatk¿n N,Dem¿rk¿ran MA,Cengiz F,¿im¿ek C,Postac¿ H,Vardar E,Durusoy R||Breast cancer (Tokyo, Japan) (22:374)||2015|
A comprehensive comparative analysis of the histomorphological features of ALK-rearranged lung adenocarcinoma based on driver oncogene mutations: frequent expression of epithelial-mesenchymal transition markers than other genotype.
MA5-14408 was used in immunohistochemistry to perform a detailed comparative analysis of ALK-rearranged and ALK-negative lung adenocarcinomas
|Kim H,Jang SJ,Chung DH,Yoo SB,Sun P,Jin Y,Nam KH,Paik JH,Chung JH||PloS one (8:null)||2013|
Epithelial-mesenchymal transition in breast cancer correlates with high histological grade and triple-negative phenotype.
MA5-14408 was used in immunohistochemistry to study the correlation of epithelial-mesenchymal transition with triple negative phenotype and high histological grade in breast cancer
|Jeong H,Ryu YJ,An J,Lee Y,Kim A||Histopathology (60:E87)||2012|
Down-regulation of microRNAs of the miR-200 family and miR-205, and an altered expression of classic and desmosomal cadherins in spindle cell carcinoma of the head and neck--hallmark of epithelial-mesenchymal transition.
MA5-14408 was used in immunohistochemistry to study the downregulation of specific microRNAs and altered expression of cadherins in spindle cell carcinoma of the head and neck
|Zidar N,Bo¿tjan¿i¿ E,Gale N,Kojc N,Poljak M,Glava¿ D,Cardesa A||Human pathology (42:482)||2011|
Apoptosis in the developing human heart resembles apoptosis in epithelial tissues.
MA5-14408 was used in immunohistochemistry to investigate the process of apoptosis in human heart during development
|Jer¿e M,Zidar N||Cell and tissue research (343:537)||2011|
GLI2-mediated melanoma invasion and metastasis.
MA5-14408 was used in immunohistochemistry to study the role of the Hedgehog pathway mediator GLI2 in regulating melanoma invasion and metastasis
|Alexaki VI,Javelaud D,Van Kempen LC,Mohammad KS,Dennler S,Luciani F,Hoek KS,Juàrez P,Goydos JS,Fournier PJ,Sibon C,Bertolotto C,Verrecchia F,Saule S,Delmas V,Ballotti R,Larue L,Saiag P,Guise TA,Mauviel A||Journal of the National Cancer Institute (102:1148)||2010|
An immunohistochemical study of colon adenomas and carcinomas: E-cadherin, Syndecan-1, Ets-1.
MA5-14408 was used in immunohistochemistry to investigate the immunohistochemical differences between colon adenomas and carcinomas
|Pap Z,Pávai Z,Dénes L,Kovalszky I,Jung J||Pathology oncology research : POR (15:579)||2009|
Cadherin-catenin complex and transcription factor Snail-1 in spindle cell carcinoma of the head and neck.
MA5-14408 was used in immunohistochemistry to examine the role of transcription factor Snail-1 in spindle cell carcinoma of the head and neck
|Zidar N,Gale N,Kojc N,Volavsek M,Cardesa A,Alos L,Höfler H,Blechschmidt K,Becker KF||Virchows Archiv : an international journal of pathology (453:267)||2008|
Immunohistochemical expression of CD44 standard and E-cadherin in atypical leiomyoma and leiomyosarcoma of the uterus.
MA5-14408 was used in immunohistochemistry - paraffin section to assess the diagnostic role of CD44 and E-cadherin in atypical leiomyoma and leiomyosarcoma.
|Kaygusuz EI||Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology (35:279)||2015|
The effects of sonic hedgehog signaling pathway components on non-small-cell lung cancer progression and clinical outcome.
MA5-14408 was used in immunohistochemistry - paraffin section to study non-small-cell lung cancer progression and clinical outcome by the effects of sonic hedgehog signaling pathway components
|Hwang J,Kang MH,Yoo YA,Quan YH,Kim HK,Oh SC,Choi YH||World journal of surgical oncology (12:null)||2014|