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|Tested species reactivity||Human|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human breast tumor cell line MCF-7.|
|Purification||Ammonium sulfate precipitation, Ion-exchange chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Inhibition Assays (IA)||200 ug/ml|
|Immunocytochemistry (ICC)||2-5 µg/ml|
|Immunofluorescence (IF)||2-5 ug/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 8 publications below|
|Western Blot (WB)||See 11 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 7 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 2 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunofluorescence (IF)||See 4 publications below|
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lysophosphatidic Acid Initiates Epithelial to Mesenchymal Transition and Induces β-Catenin-mediated Transcription in Epithelial Ovarian Carcinoma.
13-1700 was used in immunocytochemistry to assess the involvement of lysophosphatidic acid in epithelial ovarian carcinoma
|Burkhalter RJ,Westfall SD,Liu Y,Stack MS||The Journal of biological chemistry (290:22143)||2015|
NLRP7 and KHDC3L, the two maternal-effect proteins responsible for recurrent hydatidiform moles, co-localize to the oocyte cytoskeleton.
13-1700 was used in immunocytochemistry to determine the localization of NLRP7 and KHDC3L in human oocytes and preimplantation embryos
|Akoury E,Zhang L,Ao A,Slim R||Human reproduction (Oxford, England) (30:159)||2015|
Helicobacter pylori dwelling on the apical surface of gastrointestinal epithelium damages the mucosal barrier through direct contact.
13-1700 was used in immunocytochemistry and western blot to study the localization of Helicobacter pylori on the apical surface of gastrointestinal epithelium and its damaging effect on the mucosal barrier
|Zhang C,Zhang H,Yu L,Cao Y||Helicobacter (19:330)||2014|
HNF4α induced chemosensitivity to oxaliplatin and 5-FU mediated by OCT1 and CNT3 in renal cell carcinoma.
13-1700 was used in immunocytochemistry to study the effect of HNF4alpha on the chemosensitivity to oxaliplatin and 5-FU in renal cell carcinoma
|Hagos Y,Wegner W,Kuehne A,Floerl S,Marada VV,Burckhardt G,Henjakovic M||Journal of pharmaceutical sciences (103:3326)||2014|
Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.
13-1700 was used in immunocytochemistry and western blot to develop and characterize a model for Hailey-Hailey disease using normal keratinocytes.
|Raiko L,Siljamäki E,Mahoney MG,Putaala H,Suominen E,Peltonen J,Peltonen S||Experimental dermatology (21:586)||2012|
The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma.
13-1700 was used in immunocytochemistry to investigate the involvement of the tumor microenvironment in the epigenetic control of genes critical to cancer progression.
|DesRochers TM,Shamis Y,Alt-Holland A,Kudo Y,Takata T,Wang G,Jackson-Grusby L,Garlick JA||Epigenetics (7:34)||2012|
Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.
||Govindarajan R,Zhao S,Song XH,Guo RJ,Wheelock M,Johnson KR,Mehta PP||The Journal of biological chemistry (277:50087)||2002|
ROCK and Dia have opposing effects on adherens junctions downstream of Rho.
13-1700 was used in immunocytochemistry and western blot to study the regulation of adherens junctions.
|Sahai E,Marshall CJ||Nature cell biology (4:408)||2002|
E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion.
13-1700 was used in western blot to identify serines in the beta-catenin-binding domain of E-cadherin that are phosphorylated
|McEwen AE,Maher MT,Mo R,Gottardi CJ||Molecular biology of the cell (25:2365)||2014|
A soluble form of the giant cadherin Fat1 is released from pancreatic cancer cells by ADAM10 mediated ectodomain shedding.
13-1700 was used in western blot to study the release of soluble giant cadherin Fat1 from pancreatic cancer cells
|Wojtalewicz N,Sadeqzadeh E,Weiß JV,Tehrani MM,Klein-Scory S,Hahn S,Schmiegel W,Warnken U,Schnölzer M,de Bock CE,Thorne RF,Schwarte-Waldhoff I||PloS one (9:null)||2014|
Snail cooperates with KrasG12D to promote pancreatic fibrosis.
13-1700 was used in western blot to elucidate the role of Snail in pancreatic cancer development.
|Shields MA,Ebine K,Sahai V,Kumar K,Siddiqui K,Hwang RF,Grippo PJ,Munshi HG||Molecular cancer research : MCR (11:1078)||2013|
EGF promotes the shedding of soluble E-cadherin in an ADAM10-dependent manner in prostate epithelial cells.
13-1700 was used in western blot to study the EGF-induced, ADAM0-dependent shedding of E-cadherin by prostate epithelial cells.
|Grabowska MM,Sandhu B,Day ML||Cellular signalling (24:532)||2012|
Determinants of response to epidermal growth factor receptor tyrosine kinase inhibition in squamous cell carcinoma of the head and neck.
13-1700 was used in western blot to assess the sensitivity of 8 squamous cell carcinoma of the head and neck cell lines to gefitinib.
|Rogers SJ,Box C,Chambers P,Barbachano Y,Nutting CM,Rh¿s-Evans P,Workman P,Harrington KJ,Eccles SA||The Journal of pathology (218:122)||2009|
ABCG2 expression and side population abundance regulated by a transforming growth factor beta-directed epithelial-mesenchymal transition.
||Yin L,Castagnino P,Assoian RK||Cancer research (68:800)||2008|
Fate of E-cadherin in early RPE cultures: transient accumulation of truncated peptides at nonjunctional sites.
||Burke JM,Hong J||Investigative ophthalmology & visual science (47:3635)||2006|
The role of calpain in the proteolytic cleavage of E-cadherin in prostate and mammary epithelial cells.
||Rios-Doria J,Day KC,Kuefer R,Rashid MG,Chinnaiyan AM,Rubin MA,Day ML||The Journal of biological chemistry (278:1372)||2003|
The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF.
||Laura RP,Witt AS,Held HA,Gerstner R,Deshayes K,Koehler MF,Kosik KS,Sidhu SS,Lasky LA||The Journal of biological chemistry (277:12906)||2002|
Decreased MUC1 expression induces E-cadherin-mediated cell adhesion of breast cancer cell lines.
||Kondo K,Kohno N,Yokoyama A,Hiwada K||Cancer research (58:2014)||1998|
Association of p120, a tyrosine kinase substrate, with E-cadherin/catenin complexes.
||Shibamoto S,Hayakawa M,Takeuchi K,Hori T,Miyazawa K,Kitamura N,Johnson KR,Wheelock MJ,Matsuyoshi N,Takeichi M||The Journal of cell biology (128:949)||1995|
Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of α2β1 integrin and E-cadherin function.
13-1700 was used in flow cytometry to investigate the effect of glycosylation on tumor cell adhesion and invasion and its mechanism
|Bassagañas S,Carvalho S,Dias AM,Pérez-Garay M,Ortiz MR,Figueras J,Reis CA,Pinho SS,Peracaula R||PloS one (9:null)||2014|
Evaluation of the antitumor activity of dacomitinib in models of human bladder cancer.
13-1700 was used in immunohistochemistry - paraffin section to study the efficacy and tolerability of the irreversible tyrosine kinase inhibitor dacomitinib in human bladder cancer models.
|Grivas PD,Day KC,Karatsinides A,Paul A,Shakir N,Owainati I,Liebert M,Kunju LP,Thomas D,Hussain M,Day ML||Molecular medicine (Cambridge, Mass.) (19:367)||2014|
Visible effects of rapamycin (sirolimus) on human skin explants in vitro.
13-1700 was used in immunohistochemistry - paraffin section to examine the effects of rapamycin treatment using an organotypic culture of human skin explants.
|Peramo A,Marcelo CL||Archives of dermatological research (305:163)||2013|
Clinicopathological significance of CADM4 expression, and its correlation with expression of E-cadherin and Ki-67 in colorectal adenocarcinomas.
13-1700 was used in immunohistochemistry - paraffin section to correlate the of CADM4, E-cadherin, and Ki-67 in colorectal adenocarcinomas.
|Jang SM,Han H,Jun YJ,Jang SH,Min KW,Sim J,Ahn HI,Lee KH,Jang KS,Paik SS||Journal of clinical pathology (65:902)||2012|
Oral contraceptive and progestin-only use correlates to tissue tumor marker expression in women with cervical intraepithelial neoplasia.
13-1700 was used in immunohistochemistry - paraffin section to study the effect of contraceptive choices on cervical intraepithelial neoplasia.
|Samir R,Asplund A,Tot T,Pekar G,Hellberg D||Contraception (85:288)||2012|
Endothelial induced EMT in breast epithelial cells with stem cell properties.
13-1700 was used in immunohistochemistry - paraffin section and western blot to test in endothelial cells contribute to the epithelial to mesenchymal transition.
|Sigurdsson V,Hilmarsdottir B,Sigmundsdottir H,Fridriksdottir AJ,Ringnér M,Villadsen R,Borg A,Agnarsson BA,Petersen OW,Magnusson MK,Gudjonsson T||PloS one (6:null)||2011|
Malignant neoplasms infiltrating pseudoangiomatous' stromal hyperplasia of the breast: an unrecognized pathway of tumour spread.
13-1700 was used in immunohistochemistry - paraffin section to study the existence of novel routes of tumour spread via open channels of pseudoangiomatous stromal hyperplasia.
|Damiani S,Eusebi V,Peterse JL||Histopathology (41:208)||2002|
A transplantable human carcinoid as model for somatostatin receptor-mediated and amine transporter-mediated radionuclide uptake.
13-1700 was used in immunohistochemistry - paraffin section to characterize a human midgut carcinoid tumor after transplantation into nude mice.
|Kölby L,Bernhardt P,Ahlman H,Wängberg B,Johanson V,Wigander A,Forssell-Aronsson E,Karlsson S,Ahrén B,Stenman G,Nilsson O||The American journal of pathology (158:745)||2001|
Three-dimensional cell culturing by magnetic levitation.
13-1700 was used in immunohistochemistry to describe the magnetic levitation method of 3D cell culturing.
|Haisler WL,Timm DM,Gage JA,Tseng H,Killian TC,Souza GR||Nature protocols (8:1940)||2013|
Further evidence that E-cadherin is not a tumour suppressor gene in invasive ductal carcinoma of the breast: an immunohistochemical study.
13-1700 was used in immunohistochemistry to examine E-cadherin-negative ductal carcinoma.
|Rakha EA,Teoh TK,Lee AH,Nolan CC,Ellis IO,Green AR||Histopathology (62:695)||2013|
Morphological and immunophenotypic analysis of breast carcinomas with basal and myoepithelial differentiation.
13-1700 was used in immunohistochemistry to study the morphological and immunophenotypic features of breast carcinomas with basal and myoepithelial differentiation.
|Rakha EA,Putti TC,Abd El-Rehim DM,Paish C,Green AR,Powe DG,Lee AH,Robertson JF,Ellis IO||The Journal of pathology (208:495)||2006|
Cooperative role of E-cadherin and sialyl-Lewis X/A-deficient MUC1 in the passive dissemination of tumor emboli in inflammatory breast carcinoma.
||Alpaugh ML,Tomlinson JS,Kasraeian S,Barsky SH||Oncogene (21:3631)||2002|
Loss of membrane expression of E-cadherin in leukemic erythroblasts.
||Acs G,LiVolsi VA||Archives of pathology & laboratory medicine (125:198)||2001|
E-cadherin impairment increases cell survival through Notch-dependent upregulation of Bcl-2.
||Ferreira AC,Suriano G,Mendes N,Gomes B,Wen X,Carneiro F,Seruca R,Machado JC||Human molecular genetics (21:334)||2012|
Carcinomas in situ of the breast with indeterminate features: role of E-cadherin staining in categorization.
||Jacobs TW,Pliss N,Kouria G,Schnitt SJ||The American journal of surgical pathology (25:229)||2001|
Immunohistochemical characterization of human olfactory tissue.
13-1700 was used in immunohistochemistry - frozen section to discuss immunohistochemical analysis of the olfactory epithelium.
|Holbrook EH,Wu E,Curry WT,Lin DT,Schwob JE||The Laryngoscope (121:1687)||2011|
E-cadherin-dependent transcriptional control of apolipoprotein A-IV gene expression in intestinal epithelial cells: a role for the hepatic nuclear factor 4.
||Peignon G,Thenet S,Schreider C,Fouquet S,Ribeiro A,Dussaulx E,Chambaz J,Cardot P,Pinçon-Raymond M,Le Beyec J||The Journal of biological chemistry (281:3560)||2006|
Role for actin filament turnover and a myosin II motor in cytoskeleton-driven disassembly of the epithelial apical junctional complex.
||Ivanov AI,McCall IC,Parkos CA,Nusrat A||Molecular biology of the cell (15:2639)||2004|
Neutrophil transepithelial migration: evidence for sequential, contact-dependent signaling events and enhanced paracellular permeability independent of transjunctional migration.
||Edens HA,Levi BP,Jaye DL,Walsh S,Reaves TA,Turner JR,Nusrat A,Parkos CA||Journal of immunology (Baltimore, Md. : 1950) (169:476)||2002|
The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts.
||Braga VM,Machesky LM,Hall A,Hotchin NA||The Journal of cell biology (137:1421)||1997|
CD324, UVO, ECAD, Arc-1, LCAM, CDHE
CAM 120/80, E-Cadherin, cadherin 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, cell-CAM 120/80, epithelial cadherin, uvomorulin, Arc-1, CD324, CDHE, ECAD, LCAM, UVO, cadherin 1, E-cadherin epithelial, calcium-dependent adhesion protein, epithelial, cadherin-E