|Tested species reactivity||Bacteria|
|Published species reactivity||Bacteria|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(13) K T A E E R E I R K A Y K(27) of E. coli DnaJ.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA3-018 detects DnaJ from bacteria.
PA3-018 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~40 kDa band representing DnaJ from E. coli extract.
The PA3-018 immunogen is a synthetic peptide corresponding to residues S(13) K T A E E R E I R K A Y K(27) of E. coli DnaJ.
In prokaryotes, the heat shock response is characterized by the induction of several proteins including DnaJ, DnaK, GroEL and GroES. DnaK is a member of the HSP70 family, and like other members, exhibits ATPase activity which facilitates the release of bound proteins and disaggregation of protein complexes within the cell. Members of this protein group serve as molecular chaperones. Levels of DnaJ and DnaK are closely linked and, in E. coli, are constituents of a single operon. DnaJ regulates DnaK in several different systems. Relative to DnaK, the level of DnaJ synthesis is quite low.
Studies have shown that DnaJ and DnaK, together with GrpE, cooperatively interact with firefly luciferase as it unfolds during thermal denaturation allowing renaturation of the protein upon return to normal temperatures. Also, DnaK together with DnaJ stimulates the binding of RepA initiator protein to the origin of P1 phage in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein.
PA3-018 was used in immunoprecipitation to identify the DNA sequences of dnaJ gene in E.coli K12
|Bardwell JC,Tilly K,Craig E,King J,Zylicz M,Georgopoulos C||The Journal of biological chemistry (261:1782)||1986|