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|Tested species reactivity||Bacteria|
|Host / Isotype||Goat / IgG|
|Immunogen||Mixture of E. coli serotypes (E. coli).|
|Storage buffer||PBS, pH 7.2, with 10mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28977 detects E. coli O+K (FITC ) from bacteria samples.
PA1-28977 has been successfully used in immunofluorescence applications. Acetone fixation of the antigen source is recommended prior to staining.
The PA1-28977 immunogen is: Mixture of E. coli serotypes (E. coli).
Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.