|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-40301 detects E-Selectin from human, chimpanzee, rhesus monkey, cynomolgous monkey and baboon samples.
PA1-40301 has been successfully used in ELISA, immunohistochemistry (frozen) and Western blot applications. It is recommended that users test the reagent and determin their own optimal dilutions: the typical starting working dilution is 1:10.
The PA1-40301 imunogen is human E-Selectin.
The antibody reacts with the Lectin Domain of E-selectin, CD62-e, formerly designated Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). The antibody reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the ELAM-1 gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.