|Immunohistochemistry (IHC)||0.1-1.0 ug/ml|
|Immunoprecipitation (IP)||2-5 ug|
|Western Blot (WB)||1-3 µg/ml|
|Immunoprecipitation (IP)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 4 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human E2F-1 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1
Gene Aliases: E2F-1; E2F1; mKIAA4009; RBAP1; RBBP3; RBP3
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