|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant human E2F-1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14341 targets E2F-1 Transcription Factor in GS, IF, IHC (P), IP, and WB applications and shows reactivity with Human and mouse samples.
The MA5-14341 immunogen is recombinant human E2F-1 protein.
E2F's are DNA-binding proteins that associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 also requires DP-1 for efficient DNA-binding and transcription modification. E2F1 is proposed to be involved in several cellular processes that range from tumor suppression, cell cycle progression, and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis. These observations place E2F among a group of apoptosis inducers that includes; c-Myc, Adenovirus E1A, and HPV E7 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The relationship between E2F family members and tumor growth in colorectal adenocarcinomas: A comparative immunohistochemical study of 100 cases.
MA5-14341 was used in immunohistochemistry to study the relationship between the immunohistochemical expression of E2F1, E2F2 and E2F4 and colorectal adenocarcinoma growth
|Xanthoulis A,Kotsinas A,Tiniakos D,Fiska A,Tentes AA,Kyroudi A,Kittas C,Gorgoulis V||Applied immunohistochemistry and molecular morphology : AIMM (22:471)||2014|
Deregulated expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial tumours of the salivary glands.
MA5-14341 was used in immunohistochemistry to study the expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial salivary gland tumours
|Vékony H,Röser K,Löning T,Raaphorst FM,Leemans CR,Van der Waal I,Bloemena E||Histopathology (53:658)||2008|
Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.
MA5-14341 was used in immunohistochemistry to evaluate immunohistochemical markers of non-small cell lung cancer using unsupervised hierarchical clustering analysis
|Au NH,Cheang M,Huntsman DG,Yorida E,Coldman A,Elliott WM,Bebb G,Flint J,English J,Gilks CB,Grimes HL||The Journal of pathology (204:101)||2004|
CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy.
MA5-14341 was used in western blot to study the protective effect of pharmacological inhibition of CDK4/6 against antracycline cytotoxicity in triple-negative breast cancer
|McClendon AK,Dean JL,Rivadeneira DB,Yu JE,Reed CA,Gao E,Farber JL,Force T,Koch WJ,Knudsen ES||Cell cycle (Georgetown, Tex.) (11:2747)||2012|
Enhanced expression of 70-kilodalton heat shock protein limits cell division in a sepsis-induced model of acute respiratory distress syndrome.
MA5-14341 was used in western blot to examine the effect of exogenous 70-kilodalton heat shock protein expression on the pathophysiology of acute respiratory distress syndrome
|Bromberg Z,Raj N,Goloubinoff P,Deutschman CS,Weiss YG||Critical care medicine (36:246)||2008|
UCN-01 suppresses E2F-1 mediated by ubiquitin-proteasome-dependent degradation.
MA5-14341 was used in western blot to investigate the mechniasm for the suppression of E2F-1 expression by UCN-01
|Hsueh CT,Wu YC,Schwartz GK||Clinical cancer research : an official journal of the American Association for Cancer Research (7:669)||2001|