Immunohistochemistry analysis of E2F-1 Transcription Factor (KH95) showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E2F-1 Transcription Factor Antibody (KH95) Mouse Monoclonal Antibody (MA514344) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant human E2F-1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14344 targets E2F-1 Transcription Factor in gel shift, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human and mouse samples.
The MA5-14344 immunogen is recombinant human E2F-1 protein.
E2F's are DNA-binding proteins that associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 also requires DP-1 for efficient DNA-binding and transcription modification. E2F1 is proposed to be involved in several cellular processes that range from tumor suppression, cell cycle progression, and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis. These observations place E2F among a group of apoptosis inducers that includes; c-Myc, Adenovirus E1A, and HPV E7 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The relationship between E2F family members and tumor growth in colorectal adenocarcinomas: A comparative immunohistochemical study of 100 cases.
MA5-14344 was used in immunohistochemistry to study the relationship between the immunohistochemical expression of E2F1, E2F2 and E2F4 and colorectal adenocarcinoma growth
|Xanthoulis A,Kotsinas A,Tiniakos D,Fiska A,Tentes AA,Kyroudi A,Kittas C,Gorgoulis V||Applied immunohistochemistry and molecular morphology : AIMM (22:471)||2014|
Deregulated expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial tumours of the salivary glands.
MA5-14344 was used in immunohistochemistry to study the expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial salivary gland tumours
|Vékony H,Röser K,Löning T,Raaphorst FM,Leemans CR,Van der Waal I,Bloemena E||Histopathology (53:658)||2008|
Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.
MA5-14344 was used in immunohistochemistry to evaluate immunohistochemical markers of non-small cell lung cancer using unsupervised hierarchical clustering analysis
|Au NH,Cheang M,Huntsman DG,Yorida E,Coldman A,Elliott WM,Bebb G,Flint J,English J,Gilks CB,Grimes HL||The Journal of pathology (204:101)||2004|
Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease.
MA5-14344 was used in immunohistochemistry - paraffin section to study the clinical manifestation of Alzheimer's disease involving cell-cycle regulation, neuronal death and repair of oxidative DNA damage
|Silva AR,Santos AC,Farfel JM,Grinberg LT,Ferretti RE,Campos AH,Cunha IW,Begnami MD,Rocha RM,Carraro DM,de Bragança Pereira CA,Jacob-Filho W,Brentani H||PloS one (9:null)||2014|
Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
MA5-14344 was used in immunohistochemistry - paraffin section to identify diagnostic and prognostic markers for glioblastoma
|Jung Y,Joo KM,Seong DH,Choi YL,Kong DS,Kim Y,Kim MH,Jin J,Suh YL,Seol HJ,Shin CS,Lee JI,Kim JH,Song SY,Nam DH||International journal of oncology (40:1122)||2012|
Impact of E2F-1 expression on clinical outcome of gastric adenocarcinoma patients with adjuvant chemoradiation therapy.
MA5-14344 was used in immunohistochemistry - paraffin section to assess expression and prognostic significance of E2F-1 and thymidylate synthase in R(0)-resected gastric adenocarcinoma patients
|Lee J,Park CK,Park JO,Lim T,Park YS,Lim HY,Lee I,Sohn TS,Noh JH,Heo JS,Kim S,Lim DH,Kim KM,Kang WK||Clinical cancer research : an official journal of the American Association for Cancer Research (14:82)||2008|
CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy.
MA5-14344 was used in western blot to study the protective effect of pharmacological inhibition of CDK4/6 against antracycline cytotoxicity in triple-negative breast cancer
|McClendon AK,Dean JL,Rivadeneira DB,Yu JE,Reed CA,Gao E,Farber JL,Force T,Koch WJ,Knudsen ES||Cell cycle (Georgetown, Tex.) (11:2747)||2012|
Enhanced expression of 70-kilodalton heat shock protein limits cell division in a sepsis-induced model of acute respiratory distress syndrome.
MA5-14344 was used in western blot to examine the effect of exogenous 70-kilodalton heat shock protein expression on the pathophysiology of acute respiratory distress syndrome
|Bromberg Z,Raj N,Goloubinoff P,Deutschman CS,Weiss YG||Critical care medicine (36:246)||2008|
|Not Applicable||Not Cited||
Overexpression of N-Myc rapidly causes acute myeloid leukemia in mice.
MA5-14344 was used in western blot to determine whether N-Myc overexpression is highly oncogenic in mouse myeloid cells
|Kawagoe H,Kandilci A,Kranenburg TA,Grosveld GC||Cancer research (67:10677)||2007|
UCN-01 suppresses E2F-1 mediated by ubiquitin-proteasome-dependent degradation.
MA5-14344 was used in western blot to investigate the mechniasm for the suppression of E2F-1 expression by UCN-01
|Hsueh CT,Wu YC,Schwartz GK||Clinical cancer research : an official journal of the American Association for Cancer Research (7:669)||2001|