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Immunohistochemistry analysis of E2F-4 Transcription Factor showing staining in the nucleus of paraffin-embedded human colon adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E2F-4 Transcription Factor Mouse Monoclonal Antibody (MA511276) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human E2F-4 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11276 targets E2F-4 Transcription Factor in GS, IF, IHC (P), and WB applications and shows reactivity with Human and Rat samples.
The MA5-11276 immunogen is recombinant human E2F-4 protein.
E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides Differential phosphorylation contributes to the microheterogeniety of E2F-4 (total aa 411-416, varies due to a trinucleotide repeat).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of isotretinoin on spermatogenesis of rats.
MA5-11276 was used in immunohistochemistry to investigate the influence of isotretinoin administration on rodent spermatogenic activity
|Gencoglan G,Tosun M||Cutaneous and ocular toxicology (30:55)||2011|
High-resolution analysis of 16q22.1 in breast carcinoma using DNA amplifiable probes (multiplex amplifiable probe hybridization technique) and immunohistochemistry.
MA5-11276 was used in immunohistochemistry to study 16q22.1 in breast carcinoma and identify potential tumor suppressor genes
|Rakha EA,Armour JA,Pinder SE,Paish CE,Ellis IO||International journal of cancer (114:720)||2005|
Classical Hodgkin lymphoma is characterized by high constitutive expression of activating transcription factor 3 (ATF3), which promotes viability of Hodgkin/Reed-Sternberg cells.
MA5-11276 was used in western blot to study the critical role of activating transcription factor 3 in the pathogenesis classical Hodgkin's lymphoma
|Janz M,Hummel M,Truss M,Wollert-Wulf B,Mathas S,Jöhrens K,Hagemeier C,Bommert K,Stein H,Dörken B,Bargou RC||Blood (107:2536)||2006|
The adenovirus E4-6/7 protein directs nuclear localization of E2F-4 via an arginine-rich motif.
MA5-11276 was used in western blot to study the role of adenovirus E4-6/7 protein in directing nuclear localization of E2F-4
|Schaley JE,Polonskaia M,Hearing P||Journal of virology (79:2301)||2005|
p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2.
MA5-11276 was used in western blot to study the role of Skp2 in the mechanism by which p107 inhibits G1/S phase progression
|Rodier G,Makris C,Coulombe P,Scime A,Nakayama K,Nakayama KI,Meloche S||The Journal of cell biology (168:55)||2005|
E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; transcription factor E2F4
2010111M04Rik; AI427446; E2F-4; E2F4