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Invitrogen
E2F3 Monoclonal Antibody (PG30)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
E2F3 Antibody (MA1-25333) in WB
Western blot was performed using Anti-E2F3 Mouse Monoclonal Antibody (Product # MA1-25333) and a 52 kDa bands corresponding to E2F3 was observed across cell line tested and increased upon treatment with Doxorubicin and Etoposide. Whole cell extracts (30 µg lysate) of U2OS (Lane 1), U2OS treated with Doxorubicin (300ng/ml for 12 Hours) (Lane 2), U2OS treated with Etoposide (6µg/ml for 12 Hours) (Lane 3) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocell... View More
Product Details
MA1-25333
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
PG30
Immunogen
Recombinant human protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
2.3 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2293325
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686C18211; E2F-3; MGC104598; RP1-177P22.2; Transcription factor E2F3
Gene Aliases: E2F-3; E2F3; KIAA0075
UniProt ID: (Human) O00716
Entrez Gene ID: (Human) 1871
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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