Invitrogen

E2F4 Monoclonal Antibody (4E2F04 (WUF10))

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

5 References

View all (12) E2F4 antibodies

Datasheet

Datasheet

Product Image Gallery

E2F4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of E2F-4 Transcription Factor showing staining in the nucleus of paraffin-embedded human colon adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E2F-4 Transcription Factor Mouse Monoclonal Antibody (Product # MA5-11276) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

E2F4 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human tonsil stained with E2F-4 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.

E2F4 Antibody in Western Blot (WB) — Knockdown of E2F4 was achieved by transfecting HCT 116 with E2F4 specific siRNAs (Silencer® select Product # s4414). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the E2F4 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with E2F4 Monoclonal Antibody (4E2F04 (WUF10)) (Product # MA5-11276, 2 µg/ml) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to E2F4.

E2F4 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HCT 116 cells were transfected with E2F4 siRNA and loss of signal was observed in Western Blot using E2F4 Monoclonal Antibody (4E2F04 (WUF10)) (Product # MA5-11276). {KD}

Product Details

Tested Applications

Dilution

Gel Shift (GS)

1mg/mL

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Western Blot (WB)

2 µg/ml

Published Applications

Immunohistochemistry (IHC)

See 2 publications below

Western Blot (WB)

See 3 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E2F04 (WUF10)

Immunogen

Recombinant human E2F-4 protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10984014

Product Specific Information

MA5-11276 targets E2F-4 Transcription Factor in GS, IF, IHC (P), and WB applications and shows reactivity with Human and Rat samples.

The MA5-11276 immunogen is recombinant human E2F-4 protein.

Target Information

E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides Differential phosphorylation contributes to the microheterogeniety of E2F-4 (total aa 411-416, varies due to a trinucleotide repeat).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.