Invitrogen

E2F4 Monoclonal Antibody (4E2F04 (WUF10))

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (26) E2F4 antibodies

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E2F4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of E2F-4 Transcription Factor showing staining in the nucleus of paraffin-embedded human colon adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E2F-4 Transcription Factor Mouse Monoclonal Antibody (Product # MA5-11276) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

E2F4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of E2F-4 Transcription Factor showing staining in the nucleus of paraffin-embedded mouse spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E2F-4 Transcription Factor Mouse Monoclonal Antibody (Product # MA5-11276) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

E2F4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of E2F-4 Transcription Factor showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E2F-4 Transcription Factor Mouse Monoclonal Antibody (Product # MA5-11276) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

E2F4 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human tonsil stained with E2F-4 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.

E2F4 Antibody in Western Blot (WB) — Knockdown of E2F4 was achieved by transfecting HCT 116 with E2F4 specific siRNAs (Silencer® select Product # s4414). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the E2F4 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with E2F4 Monoclonal Antibody (4E2F04 (WUF10)) (Product # MA5-11276, 2 µg/ml) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to E2F4.

E2F4 Antibody in Western Blot (WB) — Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HCT 116 (Lane 1), SW-480 (Lane 2), HT-29 (Lane 3), COLO 205 (Lane 4), MDA-MB-231 (Lane 5) and Raji (Lane 6). The blot was probed with Anti-E2F4 Monoclonal Antibody (Product # MA5-11276, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 50 kDa band corresponding to E2F4 was observed across the cell lines tested.

E2F4 Antibody in Western Blot (WB) — Western blot of E2F-4 Transcription Factor using E2F-4 Transcription Factor Monoclonal Antibody (Product # MA5-11276) on Raji Cells.

E2F4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HCT 116 cells were transfected with E2F4 siRNA and loss of signal was observed in Western Blot using E2F4 Monoclonal Antibody (4E2F04 (WUF10)) (Product # MA5-11276). {KD}

Product Details

MA5-11276

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/ml	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20	-
Gel Shift (GS)	1mg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	4E2F04 (WUF10)
Immunogen	Recombinant human E2F-4 protein
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10984014

Product Specific Information

MA5-11276 targets E2F-4 Transcription Factor in GS, IF, IHC (P), and WB applications and shows reactivity with Human and Rat samples.

The MA5-11276 immunogen is recombinant human E2F-4 protein.

Target Information

E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides Differential phosphorylation contributes to the microheterogeniety of E2F-4 (total aa 411-416, varies due to a trinucleotide repeat).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of isotretinoin on spermatogenesis of rats.

Cutaneous and ocular toxicology

Gencoglan G,Tosun M

MA5-11276 was used in immunohistochemistry to investigate the influence of isotretinoin administration on rodent spermatogenic activity

Tue Mar 01 00:00:00 EST 2011

Classical Hodgkin lymphoma is characterized by high constitutive expression of activating transcription factor 3 (ATF3), which promotes viability of Hodgkin/Reed-Sternberg cells.

Blood

Janz M,Hummel M,Truss M,Wollert-Wulf B,Mathas S,Jöhrens K,Hagemeier C,Bommert K,Stein H,Dörken B,Bargou RC

MA5-11276 was used in western blot to study the critical role of activating transcription factor 3 in the pathogenesis classical Hodgkin's lymphoma

Wed Mar 15 00:00:00 EST 2006

High-resolution analysis of 16q22.1 in breast carcinoma using DNA amplifiable probes (multiplex amplifiable probe hybridization technique) and immunohistochemistry.

International journal of cancer

Rakha EA,Armour JA,Pinder SE,Paish CE,Ellis IO

MA5-11276 was used in immunohistochemistry to study 16q22.1 in breast carcinoma and identify potential tumor suppressor genes

Sun May 01 00:00:00 EDT 2005

The adenovirus E4-6/7 protein directs nuclear localization of E2F-4 via an arginine-rich motif.

Journal of virology

Schaley JE,Polonskaia M,Hearing P

MA5-11276 was used in western blot to study the role of adenovirus E4-6/7 protein in directing nuclear localization of E2F-4

Tue Feb 01 00:00:00 EST 2005

p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2.

The Journal of cell biology

Rodier G,Makris C,Coulombe P,Scime A,Nakayama K,Nakayama KI,Meloche S

MA5-11276 was used in western blot to study the role of Skp2 in the mechanism by which p107 inhibits G1/S phase progression

Mon Jan 03 00:00:00 EST 2005

Bioinformatics

Protein Aliases: E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; Transcription factor E2F4

Gene Aliases: 2010111M04Rik; AI427446; E2F-4; E2F4

UniProt ID: (Human) Q16254, (Mouse) Q8R0K9

Entrez Gene ID: (Human) 1874, (Rat) 100360427, (Mouse) 104394

Molecular Function: RNA metabolism protein nucleic acid metabolism protein general transcription factor

regulation of transcription involved in G1/S transition of mitotic cell cycle epithelial cell development transcription from RNA polymerase II promoter cell volume homeostasis DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest blood circulation organ morphogenesis regulation of cell proliferation motile cilium assembly positive regulation of transcription from RNA polymerase II promoter centriole assembly multi-ciliated epithelial cell differentiation transcription, DNA-templated regulation of cell size cilium assembly mitotic cell cycle regulation of transcription, DNA-templated cell cycle cell projection organization regulation of cell cycle

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein domain specific binding promoter-specific chromatin binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding